Human PADI2 Antibody

Catalog # Availability Size / Price Qty
AF7257
AF7257-SP
PADI2 in Human Brain.
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Human PADI2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PADI2 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PADI2
Met1-Ala115
Accession # Q9Y2J8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry PADI2 in Human Brain. View Larger

PADI2 in Human Brain. PADI2 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain (cortex) using Sheep Anti-Human PADI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7257) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PADI2

PADI2 (Protein-arginine deiminase type 2; also PAD2, PDI2 and PAD-H19) is a 72-76 kDa member of the PAD family of proteins. It is widely expressed, being found in macrophages, astrocytes, select neurons, skeletal muscle and bronchial epithelial cells, and keratinocytes. PADI2 catalyzes the Ca++-dependent conversion of guanidino groups (NH2-C[=NH]-NH−) to ureido groups (NH2-C[=O]-NH-) on peptidylArg, a posttranslational process known as citrullination. Human PADI2 is 665 amino acids (aa) in length. It contains an N-terminal PAD domain (aa 1-114), a middle PAD-M domain (aa 116-274), and a catalytic region (aa 279-665). Over aa 1-115, human PADI2 shares 90% aa sequence identity with mouse PADI2.

Long Name
Peptidyl Arginine Deiminase type II
Entrez Gene IDs
11240 (Human); 18600 (Mouse); 29511 (Rat)
Alternate Names
EC 3.5.3.15; KIAA0994PDI2PAD2; PAD2; PAD-H19; PADI2; PDI2; peptidlyarginine deiminase type II; peptidyl arginine deiminase, type II; Peptidylarginine Deiminase II; protein arginine deiminase; Protein-arginine deiminase type II; protein-arginine deiminase type-2

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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