Human PAWR Antibody

Catalog # Availability Size / Price Qty
AF6885
AF6885-SP
PAWR in LNCaP Human Cell Line.
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Human PAWR Antibody Summary

Species Reactivity
Human
Specificity
Detects human PAWR in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PAWR
Arg2-Ala121
Accession # CAD88640
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry PAWR in LNCaP Human Cell Line. View Larger

PAWR in LNCaP Human Cell Line. PAWR was detected in immersion fixed LNCaP human prostate cancer cell line using Sheep Anti-Human PAWR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAWR

PAWR (PRKC Apoptosis WT1 Regulator protein; also PAR-4) is an intracellular, 38-42 kDa pro-apoptotic protein. It is widely expressed, and serves multiple functions. WT1 protein is both a transcriptional activator and repressor. When complexed to PAWR, WT1 activation function is repressed, while its repressor activity is enhanced. Thus, PAWR generates transcriptional repression. PAWR also binds to the atypical lambda PKC and zeta PKC isotypes. Such binding inhibits PKC avtivity, blocks cell division and MAPK activation, and promotes Fas-mediated cell apoptosis. Finally, in neurons, PAWR binds to BACE1, promoting the cleavage of APP. Human PAWR is 340 amino acids (aa) in length. It contains an Ala-rich region (aa 49-120), an NLS (aa 145-161), one coiled-coil region (aa 186-206), and a Leu-zipper domain (aa 300-340). There are at least five utilized Ser/Thr phosphorylation sites. PAWR forms noncovalent homodimers and is reported to homooligomerize. There is one potential splice form that shows a three aa substitution for aa 173-340, and a P-P-A-R substitution for A102P103. Full-length PAWR shares 78% aa identity with mouse PAWR.

           

Long Name
PRKC, Apoptosis, WT1, Regulator
Entrez Gene IDs
5074 (Human); 114774 (Mouse); 64513 (Rat)
Alternate Names
PAR4; PAWR; WT1 Interacting Protein

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