Human PD-1 Biotinylated Antibody

(9 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD-1 is observed and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human PD-1
    Leu25-Gln167
    Accession # Q8IX89
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
    • Human PD-1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human PD‑1 Antibody (Catalog # AF1086)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human PD‑1 Biotinylated Antibody (Catalog # BAF1086)
  • ELISA Standard
     
    Recombinant Human PD-1 Fc Chimera Protein, CF (Catalog # 1086-PD)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PD‑1 in Human PBMCs treated with PHA by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 μg/mL PHA overnight were stained with Goat Anti-Human PD‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1086) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody(Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # BAF108). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-1

Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1) (2). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD-1 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10, and IFN-gamma. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (2, 3).

Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2 (also known as B7-DC), have been identified as PD-1 ligands. Unlike other B7 family proteins, both PD‑L1 and PD‑L2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens (4). The wide expression of PD-L1 and PD-L2 and the inhibitor effects on PD-1 ligation indicate that PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases (2).

The human PD-1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immuno-receptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 60% aa sequence identity (4).

  • References:
    1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
    2. Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
    3. Latchman, Y. et al. (2001) Nature Immun. 2:261.
    4. Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.
  • Long Name:
    Programmed Death-1
  • Entrez Gene IDs:
    5133 (Human); 18566 (Mouse); 301626 (Rat); 486213 (Canine); 102123659 (Cynomolgus Monkey)
  • Alternate Names:
    CD279 antigen; CD279; hPD-1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Immunohistochemical Analysis of PD-L1 Expression in Canine Malignant Cancers and PD-1 Expression on Lymphocytes in Canine Oral Melanoma
    Authors: Naoya Maekawa
    PLoS ONE, 2016;11(6):e0157176.
    Species: Canine
    Sample Type: Whole Cells
    Application: Flow
  2. Loss of circulating CD4 T cells with B cell helper function during chronic HIV infection.
    Authors: Boswell K, Paris R, Boritz E, Ambrozak D, Yamamoto T, Darko S, Wloka K, Wheatley A, Narpala S, McDermott A, Roederer M, Haubrich R, Connors M, Ake J, Douek D, Kim J, Petrovas C, Koup R
    PLoS Pathog, 2014;10(1):e1003853.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Type I interferon responses in rhesus macaques prevent SIV infection and slow disease progression.
    Authors: Sandler N, Bosinger S, Estes J, Zhu R, Tharp G, Boritz E, Levin D, Wijeyesinghe S, Makamdop K, Del Prete G, Hill B, Timmer J, Reiss E, Yarden G, Darko S, Contijoch E, Todd J, Silvestri G, Nason M, Norgren R, Keele B, Rao S, Langer J, Lifson J, Schreiber G, Douek D
    Nature, 2014;511(7511):601-5.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  4. Programmed death 1-mediated T cell exhaustion during visceral leishmaniasis impairs phagocyte function.
    Authors: Esch K, Juelsgaard R, Martinez P, Jones D, Petersen C
    J Immunol, 2013;191(11):5542-50.
    Species: Canine
    Sample Type: Whole Cells
    Application: Flow
  5. CD4 T follicular helper cell dynamics during SIV infection.
    J. Clin. Invest., 2012;122(9):3281-94.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  6. Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones in rhesus macaques during acute infection.
    Authors: Minang JT, Trivett MT, Bolton DL, Trubey CM, Estes JD, Li Y, Smedley J, Pung R, Rosati M, Jalah R, Pavlakis GN, Felber BK, Piatak M, Roederer M, Lifson JD, Ott DE, Ohlen C
    J. Immunol., 2010;184(1):315-26.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  7. Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques.
    Authors: Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M, O'Connor D, Lifson JD, Roederer M, Ohlen C
    J. Immunol., 2010;184(1):303-14.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  8. Activation drives PD-1 expression during vaccine-specific proliferation and following lentiviral infection in macaques.
    Authors: Hokey DA, Johnson FB, Smith J, Weber JL, Yan J, Hirao L, Boyer JD, Lewis MG, Makedonas G, Betts MR, Weiner DB
    Eur. J. Immunol., 2008;38(5):1435-45.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  9. Programmed Death-1: from gene to protein in autoimmune human myasthenia gravis.
    Authors: Sakthivel P, Ramanujam R, Wang XB, Pirskanen R, Lefvert AK
    J. Neuroimmunol., 2007;193(1):149-55.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Staining Reagents
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Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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