Human PDCD4 Antibody

  
  • Specificity
    Detects human PDCD4 in direct ELISAs.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PDCD4
    Lys212-Pro357
    Accession # Q53EL6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
PDCD4 in MCF-7 Human Cell Line. PDCD4 was detected in immersion fixed MCF‑7 human breast cancer cell line using Sheep Anti-Human PDCD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7019) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
PDCD4 in Human Stomach. PDCD4 was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human PDCD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7019) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDCD4

PDCD4 (Programmed cell death protein 4; also H731) is a 54-64 kDa member of the PDCD4 family of molecules. It is widely expressed, being found in mammary epithelium, CD34+ bone marrow progenitor cells, fibroblasts and keratinocytes. PDCD4 is both cytoplasmic and nuclear. In the cytoplasm, it blocks protein translation by binding to eIF4A, an act that dissociates eIF4G and mRNA from eIF4A. In the nucleus, it seems to block transcription of select genes, one of which is MAP4K1, a key enzyme in the AP-1-mediated transcription pathway. Human PDCD4 is 469 amino acids (aa) in length. It contains an NLS (aa 58-64), an MI domain (aa 163-284), another NLS (aa 241-250) and a second MI domain (aa 326-449). There are at least seven utilized Ser/Thr phosphorylation sites and one Tyr phosphorylation site. There are two potential splice forms, one that contains a deletion of aa 15-28, and a second that may show a three aa substitution for aa 2-15. Over aa 212-357, human PDCD4 shares 98% aa identity with mouse PDCD4.

  • Long Name:
    Programmed Cell Death 4
  • Entrez Gene IDs:
    27250 (Human); 18569 (Mouse); 64031 (Rat)
  • Alternate Names:
    H731; Protein 197/15a; H731nuclear antigen H731; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; programmed cell death 4 (neoplastic transformation inhibitor); programmed cell death protein 4; Protein 197/15a
Related Research Areas
Cell and Tissue Staining Kits
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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019
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Anti-Sheep HRP-AEC Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 5
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Antigen Retrieval Reagent-Basic

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Mounting Medium

CTS011 1
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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DAB Enhancer

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NorthernLights Guard Mounting Media

NL996
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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 3
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF016 5  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 2
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 2
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Sheep IgG (H+L) PE‑conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC‑conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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Donkey Anti-Sheep IgG (H+L) Antibody

D-501-C-ABS
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