Human PDGF-D Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human PDGF‑D in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PDGF, rhPDGF-AA, rhPDGF-AB, rhPDGF-BB, rhPDGF-C, or recombinant rat PDGF-AB is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 680018
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human PDGF‑DD
    Ser250-Arg370
    Accession # Q9GZP0.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize PDGF‑DD-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E. W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND50) is typically 1.5-7.5 µg/mL in the presence of 0.75 ug/mL Recombinant Human PDGF‑DD.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by PDGF‑DD and Neutralization by Human PDGF‑D Antibody. Recombinant Human PDGF‑DD (Catalog # 1159-SB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑DD (0.75 ug/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF‑D Monoclonal Antibody (Catalog # MAB1159). The ND50 is typically 1.5‑7.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF-D
The platelet-derived growth factor (PDGF) family consists of four disulfide-linked homodimers and one heterodimer (PDGF-AB). These proteins regulate diverse cellular functions through interactions with PDGF R alpha and R beta (1, 2). Mature PDGF-DD associates with PDGF R beta and triggers signaling through PDGF R beta homodimers and PDGF R alpha / beta heterodimers (3‑5). The human PDGF-DD cDNA encodes a 370 amino acid (aa) precursor that includes a 23 aa signal sequence, one CUB domain, and one PDGF/VEGF domain (3, 4). The PDGF/VEGF domain shares 27‑35% aa sequence identity with the corresponding regions of other PDGF family members. Human PDGF-DD shares 87% aa sequence identity with mouse and rat PDGF-DD. PDGF-DD is secreted as a100 kDa latent homodimer which is activated by proteolysis to release a 35 kDa bioactive protein containing the PDGF/VEGF homology domain (3,4,6,7). A splice variant of PDGF-DD has a 6 aa deletion near the N-terminus. A 72 aa deletion within the PDGF/VEGF domain generates an inactive protein in mouse but has not been detected in human (8). PDGF-DD is widely expressed in embryonic and adult tissues (3, 9, 10), and PDGF R beta is expressed in a generally complementary pattern (9, 11, 12). PDGF-DD functions as a growth factor for renal artery smooth muscle cells and lens epithelial cells, and as a macrophage chemoattractant (5, 9‑11). PDGF-DD is overexpressed in and contributes to several disease states, including renal and hepatic fibrosis, mesangial proliferative glomerulopathy, pulmonary lymphoid infiltration, and many cancers (6, 11‑15). PDGF-DD functions in both paracrine and autocrine manners (6, 7, 14).
  • References:
    1. Reigstad, L.J. et al. (2005) FEBS J. 272:5723.
    2. Fredriksson, L. et al. (2004) Cytokine Growth Factor Rev. 15:197.
    3. LaRochelle, W.J. et al. (2001) Nat. Cell Biol. 3:517.
    4. Bergsten, E. et al. (2001) Nat. Cell Biol. 3:512.
    5. Uutela, M. et al. (2004) Blood 104:3198.
    6. Ustach, C.V. and H-R.C. Kim (2005) Mol. Cell. Biol. 25:6279. 
    7. Ustach, C.V. et al. (2004) Canc. Res. 64:1722.
    8. Zhuo, Y. et al. (2003) Biochem. Biophys. Res. Commun. 308:126.
    9. Changsirikulchai, S. et al. (2002) Kid. Int. 62:2043.
    10. Ray, S. et al. (2005) J. Biol. Chem. 280:8494.
    11. Hudkins, K.L. et al. (2004) J. Am. Soc. Nephrol. 15:286.
    12. Lokker, N.A. et al. (2002) Canc. Res. 62:3729.
    13. Taneda, S. et al. (2003) J. Am. Soc. Nephrol. 14:2544.
    14. LaRochelle, W.J. et al. (2002) Canc. Res. 62:2468.
    15. Xu, L. et al. (2005) Canc. Res. 65:5711.
  • Long Name:
    Platelet-derived Growth Factor D
  • Entrez Gene IDs:
    80310 (Human); 71785 (Mouse)
  • Alternate Names:
    IEGFMSTP036; Iris-expressed growth factor; PDGFD; PDGF-D; platelet derived growth factor D; SCDGF-BMGC26867; SCDGFBplatelet-derived growth factor D; spinal cord derived growth factor B; Spinal cord-derived growth factor B; spinal cord-derived growth factor-B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Phenotypic plasticity in normal breast derived epithelial cells.
    Authors: Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, Blosser R, Radovich M, Lam C, Vaughan M, Herbert B, Clare S
    BMC Cell Biol, 2014;15(0):20.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Frozen
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Mouse IgG1 Isotype Control

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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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