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Human PDGF-D Antibody

Catalog #: MAB1159
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1159-SP
MAB1159-100
MAB1159-500
Cell Proliferation Induced by PDGF‑DD and Neutralization by Human PDGF‑D Antibody.
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Citations (2)
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Human PDGF-D Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF‑D in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PDGF, rhPDGF-AA, rhPDGF-AB, rhPDGF-BB, rhPDGF-C, or recombinant rat PDGF-AB is observed.
Source
Monoclonal Mouse IgG1 Clone # 680018
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PDGF‑DD
Ser250-Arg370
Accession # Q9GZP0.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize PDGF‑DD-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E. W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND50) is typically 1.5-7.5 µg/mL in the presence of 0.75 ug/mL Recombinant Human PDGF‑DD.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by PDGF‑DD and Neutralization by Human PDGF‑D Antibody. View Larger

Cell Proliferation Induced by PDGF‑DD and Neutralization by Human PDGF‑D Antibody. Recombinant Human PDGF-DD (Catalog # 1159-SB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-DD (0.75 ug/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF-D Monoclonal Antibody (Catalog # MAB1159). The ND50 is typically 1.5-7.5 µg/mL.

Immunocytochemistry/ Immunofluorescence Detection of PDGF-D by Immunocytochemistry/ Immunofluorescence View Larger

Detection of PDGF-D by Immunocytochemistry/ Immunofluorescence Antioxidant effect of enriched proteins in SHED-CM. (A) Representative immunofluorescence images of MitoSOX analysis with nuclear DAPI staining of human acinar cell line 24 h after irradiation. Scale bar: 100 μm. (B) Quantitative analysis of the fluorescence intensities of MitoSOX analysis. The average fluorescence intensities were significantly higher in the irradiated cells treated with SHED-CM together with anti-PDGF-D antibodies or anti-HGF antibodies than those treated with SHED-CM alone. The antioxidant effect of SHED-CM was most strongly inhibited after treatment with four neutralizing antibodies together. Comparisons between groups were analyzed using an ANOVA with Tukey's multiple comparison test. Data are represented as mean ± SD (**p < 0.01, ***p < 0.001). CM, conditioned medium; HGF, hepatocyte growth factor; Fibro, fibroblast; PDGF, platelet-derived growth factor; SHED, stem cells from human exfoliated deciduous teeth. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36792628), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of PDGF-D by Immunocytochemistry/ Immunofluorescence View Larger

Detection of PDGF-D by Immunocytochemistry/ Immunofluorescence Antioxidant effect of enriched proteins in SHED-CM. (A) Representative immunofluorescence images of MitoSOX analysis with nuclear DAPI staining of human acinar cell line 24 h after irradiation. Scale bar: 100 μm. (B) Quantitative analysis of the fluorescence intensities of MitoSOX analysis. The average fluorescence intensities were significantly higher in the irradiated cells treated with SHED-CM together with anti-PDGF-D antibodies or anti-HGF antibodies than those treated with SHED-CM alone. The antioxidant effect of SHED-CM was most strongly inhibited after treatment with four neutralizing antibodies together. Comparisons between groups were analyzed using an ANOVA with Tukey's multiple comparison test. Data are represented as mean ± SD (**p < 0.01, ***p < 0.001). CM, conditioned medium; HGF, hepatocyte growth factor; Fibro, fibroblast; PDGF, platelet-derived growth factor; SHED, stem cells from human exfoliated deciduous teeth. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36792628), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-D

The platelet-derived growth factor (PDGF) family consists of four disulfide-linked homodimers and one heterodimer (PDGF-AB). These proteins regulate diverse cellular functions through interactions with PDGF R alpha and R beta (1, 2). Mature PDGF-DD associates with PDGF R beta and triggers signaling through PDGF R beta homodimers and PDGF R alpha / beta heterodimers (3‑5). The human PDGF-DD cDNA encodes a 370 amino acid (aa) precursor that includes a 23 aa signal sequence, one CUB domain, and one PDGF/VEGF domain (3, 4). The PDGF/VEGF domain shares 27‑35% aa sequence identity with the corresponding regions of other PDGF family members. Human PDGF-DD shares 87% aa sequence identity with mouse and rat PDGF-DD. PDGF-DD is secreted as a100 kDa latent homodimer which is activated by proteolysis to release a 35 kDa bioactive protein containing the PDGF/VEGF homology domain (3,4,6,7). A splice variant of PDGF-DD has a 6 aa deletion near the N-terminus. A 72 aa deletion within the PDGF/VEGF domain generates an inactive protein in mouse but has not been detected in human (8). PDGF-DD is widely expressed in embryonic and adult tissues (3, 9, 10), and PDGF R beta is expressed in a generally complementary pattern (9, 11, 12). PDGF-DD functions as a growth factor for renal artery smooth muscle cells and lens epithelial cells, and as a macrophage chemoattractant (5, 9‑11). PDGF-DD is overexpressed in and contributes to several disease states, including renal and hepatic fibrosis, mesangial proliferative glomerulopathy, pulmonary lymphoid infiltration, and many cancers (6, 11‑15). PDGF-DD functions in both paracrine and autocrine manners (6, 7, 14).

References
  1. Reigstad, L.J. et al. (2005) FEBS J. 272:5723.
  2. Fredriksson, L. et al. (2004) Cytokine Growth Factor Rev. 15:197.
  3. LaRochelle, W.J. et al. (2001) Nat. Cell Biol. 3:517.
  4. Bergsten, E. et al. (2001) Nat. Cell Biol. 3:512.
  5. Uutela, M. et al. (2004) Blood 104:3198.
  6. Ustach, C.V. and H-R.C. Kim (2005) Mol. Cell. Biol. 25:6279. 
  7. Ustach, C.V. et al. (2004) Canc. Res. 64:1722.
  8. Zhuo, Y. et al. (2003) Biochem. Biophys. Res. Commun. 308:126.
  9. Changsirikulchai, S. et al. (2002) Kid. Int. 62:2043.
  10. Ray, S. et al. (2005) J. Biol. Chem. 280:8494.
  11. Hudkins, K.L. et al. (2004) J. Am. Soc. Nephrol. 15:286.
  12. Lokker, N.A. et al. (2002) Canc. Res. 62:3729.
  13. Taneda, S. et al. (2003) J. Am. Soc. Nephrol. 14:2544.
  14. LaRochelle, W.J. et al. (2002) Canc. Res. 62:2468.
  15. Xu, L. et al. (2005) Canc. Res. 65:5711.
Long Name
Platelet-derived Growth Factor D
Entrez Gene IDs
80310 (Human); 71785 (Mouse)
Alternate Names
IEGFMSTP036; Iris-expressed growth factor; PDGFD; PDGF-D; platelet derived growth factor D; SCDGF-BMGC26867; SCDGFBplatelet-derived growth factor D; spinal cord derived growth factor B; Spinal cord-derived growth factor B; spinal cord-derived growth factor-B

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Citations for Human PDGF-D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Phenotypic plasticity in normal breast derived epithelial cells.
    Authors: Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, Blosser R, Radovich M, Lam C, Vaughan M, Herbert B, Clare S
    BMC Cell Biol, 2014-06-10;15(0):20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for radiation-induced mouse xerostomia
    Authors: F Kano, N Hashimoto, Y Liu, L Xia, T Nishihara, W Oki, K Kawarabaya, N Mizusawa, K Aota, T Sakai, M Azuma, H Hibi, T Iwasaki, T Iwamoto, N Horimai, A Yamamoto
    Scientific Reports, 2023-02-15;13(1):2706.

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