PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated to pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a
homeodomain-containing transcription factor. During embryonic
development, PDX-1 is required for pancreas differentiation in adult
islet cells. PDX-1 regulates beta -cell specific gene expression and
function. Human and mouse PDX-1 share 88% amino acid sequence homology.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.