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Human PDX-1/IPF1 Antibody

Catalog #: AF2419
37 Citations 3 Reviews Datasheet / COA / SDS
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AF2419
AF2419-SP
Detection of Human PDX‑1/IPF1 by Western Blot.
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Product Details
Citations (37)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDX-1/IPF1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDX‑1/IPF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PDX-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PDX‑1/IPF1
Ala91-Arg283
Accession # P52945
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PDX-1/IPF1 antibody by Western Blot. View Larger

Detection of Human PDX‑1/IPF1 by Western Blot. Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX-1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Mouse PDX-1/IPF1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse PDX‑1/IPF1 by Simple WesternTM. Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX-1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry PDX-1/IPF1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry PDX-1/IPF1 antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PDX‑1/IPF1 in Human Pancreatic Cancer Tissue. PDX-1/IPF1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDX-1/IPF1

PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a homeodomain-containing transcription factor. During embryonic development, PDX-1 is required for pancreas differentiation in adult islet cells. PDX-1 regulates beta -cell specific gene expression and function. Human and mouse PDX-1 share 88% amino acid sequence homology.

Long Name
Pancreas/Duodenum Homeobox-1
Entrez Gene IDs
3651 (Human); 18609 (Mouse)
Alternate Names
Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1

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Citations for Human PDX-1/IPF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A
    Authors: A Beucher, I Miguel-Esc, D Balboa, MG De Vas, MA Maestro, J Garcia-Hur, A Bernal, R Gonzalez-F, P Vargiu, H Heyn, P Ravassard, S Ortega, J Ferrer
    Nature Cell Biology, 2022;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Methionine metabolism regulates pluripotent stem cell pluripotency and differentiation through zinc mobilization
    Authors: EZ Sim, T Enomoto, N Shiraki, N Furuta, S Kashio, T Kambe, T Tsuyama, A Arakawa, H Ozawa, M Yokoyama, M Miura, S Kume
    Cell Reports, 2022;40(3):111120.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Divergent transcriptional regulation of astrocyte reactivity across disorders
    Authors: JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew
    Nature, 2022;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models
    Authors: Y Aghazadeh, F Poon, F Sarangi, FTM Wong, ST Khan, X Sun, R Hatkar, BJ Cox, SS Nunes, MC Nostro
    Cell Stem Cell, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional &beta cells
    Authors: H Liu, R Li, HK Liao, Z Min, C Wang, Y Yu, L Shi, J Dan, A Hayek, L Martinez M, E Nuñez Deli, JC Izpisua Be
    Nature Communications, 2021;12(1):3330.
    Species: Human
    Sample Types: Whole Cellse
    Applications: IHC
  6. Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant &beta cells
    Authors: BSJ Low, CS Lim, SSL Ding, YS Tan, NHJ Ng, VG Krishnan, SF Ang, CWY Neo, CS Verma, S Hoon, SC Lim, ES Tai, AKK Teo
    Nature Communications, 2021;12(1):3133.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Generation of insulin-producing pancreatic beta cells from multiple human stem cell lines
    Authors: NJ Hogrebe, KG Maxwell, P Augsornwor, JR Millman
    Nature Protocols, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. CDKN2A-Mutated Pancreatic Ductal Organoids from Induced Pluripotent Stem Cells to Model a Cancer Predisposition Syndrome
    Authors: J Merkle, M Breunig, M Schmid, C Allgöwer, J Krüger, MK Melzer, S Bens, R Siebert, L Perkhofer, N Azoitei, T Seufferlei, S Heller, M Meier, M Müller, A Kleger, M Hohwieler
    Cancers, 2021;13(20):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. A 3D culture platform enables development of zinc-binding prodrugs for targeted proliferation of &beta cells
    Authors: K Yang, M Lee, PA Jones, SS Liu, A Zhou, J Xu, V Sreekanth, JLY Wu, L Vo, EA Lee, R Pop, Y Lee, BK Wagner, DA Melton, A Choudhary, JM Karp
    Sci Adv, 2020;6(47):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells
    Authors: R Dettmer, K Cirksena, J Münchhoff, J Kresse, U Diekmann, I Niwolik, FFR Buettner, O Naujok
    Cells, 2020;9(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Single-Cell Transcriptome Profiling Reveals &beta Cell Maturation in Stem Cell-Derived Islets after Transplantation
    Authors: P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
    Cell Rep, 2020;32(8):108067.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells
    Authors: R Dettmer, K Cirksena, J Münchhoff, J Kresse, U Diekmann, I Niwolik, FFR Buettner, O Naujok
    Cells, 2020;9(9):.
    Species: Human`
    Sample Types: Whole Cells
    Applications: ICC
  13. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
    Authors: NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
    Nat. Biotechnol., 2020;0(0):.
    Species: Human
    Sample Types: Cells
    Applications: ICC
  14. HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells
    Authors: Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban
    Nat. Immunol., 2020;21(3):274-286.
    Species: Human
    Sample Types: Cells
    Applications: ICC
  15. Generation of an INSULIN-H2B-Cherry reporter human iPSC line
    Authors: AK Blöchinger, J Siehler, K Wi beta miller, A Shahryari, I Burtscher, H Lickert
    Stem Cell Res, 2020;45(0):101797.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells
    Authors: LSW Loo, AAP Soetedjo, HH Lau, NHJ Ng, S Ghosh, L Nguyen, VG Krishnan, H Choi, X Roca, S Hoon, AKK Teo
    Cell Death Dis, 2020;11(5):378.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced
    Authors: P Seiron, A Wiberg, E Kuric, L Krogvold, FL Jahnsen, K Dahl-Jørge, O Skog, O Korsgren
    J Pathol Clin Res, 2019;5(4):248-255.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Circadian Entrainment Triggers Maturation of Human In�Vitro Islets
    Authors: JR Alvarez-Do, J Donaghey, N Rasouli, JHR Kenty, A Helman, J Charlton, JR Straubhaar, A Meissner, DA Melton
    Cell Stem Cell, 2019;26(1):108-122.e10.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation
    Authors: K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu
    Cell Rep, 2019;28(2):382-393.e7.
    Species: Human
    Sample Types: Chromatin
    Applications: Immunoprecipitation
  20. YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing ? cells
    Authors: EA Rosado-Oli, K Anderson, JH Kenty, DA Melton
    Nat Commun, 2019;10(1):1464.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Point mutations in the PDX1 transactivation domain impair human ?-cell development and function
    Authors: X Wang, M Sterr, Ansarullah, I Burtscher, A Böttcher, J Beckenbaue, J Siehler, T Meitinger, HU Häring, H Staiger, FM Cernilogar, G Schotta, M Irmler, J Beckers, CVE Wright, M Bakhti, H Lickert
    Mol Metab, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture
    Authors: U Diekmann, H Wolling, R Dettmer, I Niwolik, O Naujok, FFR Buettner
    Sci Rep, 2019;9(1):996.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. A hPSC-based platform to discover gene-environment interactions that impact human ?-cell and dopamine neuron survival
    Authors: T Zhou, TW Kim, CN Chong, L Tan, S Amin, Z Sadat Badi, S Mukherjee, Z Ghazizadeh, H Zeng, M Guo, M Crespo, T Zhang, R Kenyon, CL Robinson, E Apostolou, H Wang, JZ Xiang, T Evans, L Studer, S Chen
    Nat Commun, 2018;9(1):4815.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing
    Authors: R Wei, F Yuan, Y Wu, Y Liu, K You, Z Yang, Y Chen, A Getachew, N Wang, Y Xu, Y Zhuang, F Yang, YX Li
    Stem Cell Res, 2018;32(0):139-144.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors
    Authors: H Ida, T Akiyama, K Ishiguro, SK Goparaju, Y Nakatake, N Chikazawa-, S Sato, H Kimura, Y Yokoyama, M Nagino, MSH Ko, SBH Ko
    Stem Cell Res Ther, 2018;9(1):277.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity
    Authors: PR Tata, RD Chow, SV Saladi, A Tata, A Konkimalla, A Bara, D Montoro, LP Hariri, AR Shih, M Mino-Kenud, H Mou, S Kimura, LW Ellisen, J Rajagopal
    Dev. Cell, 2018;44(6):679-693.e5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. Functional Beta Cell Mass from Device-Encapsulated hESC-Derived Pancreatic Endoderm Achieving Metabolic Control
    Authors: T Robert, I De Mesmaek, GM Stangé, KG Suenens, Z Ling, EJ Kroon, DG Pipeleers
    Stem Cell Reports, 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC
  28. An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation
    Authors: J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski
    Cell Rep, 2017;19(2):281-294.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Testosterone improves the differentiation efficiency of insulin-producing cells from human induced pluripotent stem cells
    Authors: H Liu, D Guo, A Ruzi, Y Chen, T Pan, F Yang, J Li, K Xu, T Zhou, D Qin, YX Li
    PLoS ONE, 2017;12(6):e0179353.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  30. Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
    Authors: KF Cogger, A Sinha, F Sarangi, EC McGaugh, D Saunders, C Dorrell, S Mejia-Guer, Y Aghazadeh, JL Rourke, RA Screaton, M Grompe, PR Streeter, AC Powers, M Brissova, T Kislinger, MC Nostro
    Nat Commun, 2017;8(1):331.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Insulin-producing cells derived from 'induced pluripotent stem cells' of patients with fulminant type 1 diabetes: vulnerability to cytokine insults and increased expression of apoptosis-related genes
    Authors: Y Hosokawa, T Toyoda, K Fukui, MY Baden, M Funato, Y Kondo, T Sudo, H Iwahashi, M Kishida, C Okada, A Watanabe, I Asaka, K Osafune, A Imagawa, I Shimomura
    J Diabetes Investig, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. The Anterior-Posterior Patterning of Definitive Endoderm Generated from Human Embryonic Stem Cells Depends on the Differential Signaling of Retinoic Acid, Wnt- and BMP-Signaling
    Authors: Claudia Davenport
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  33. Repurposing cAMP-modulating medications to promote beta-cell replication.
    Authors: Zhao Z, Low Y, Armstrong N, Ryu J, Sun S, Arvanites A, Hollister-Lock J, Shah N, Weir G, Annes J
    Mol Endocrinol, 2014;28(10):1682-97.
    Species: Rat
    Sample Types: Whole Cells
    Applications: IHC
  34. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
    Cell Transplant, 2012;21(6):1321-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells.
    Authors: Sui L, Mfopou J, Geens M, Sermon K, Bouwens L
    Biochem Biophys Res Commun, 2012;426(3):380-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  36. CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
    Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H
    Stem Cells, 2011;29(4):609-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
    Authors: Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(18):6907-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human PDX-1/IPF1 Antibody

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Human PDX-1/IPF1 Antibody
By Anonymous on 04/17/2018
Application: ICC/IF Sample Tested: iPS2 human induced pluripotent stem cells Species: Human
Immunocytochemistry/Immunofluorescence Human PDX-1/IPF1 Antibody AF2419
Human PDX-1/IPF1 Antibody
By Colin Plumb on 07/08/2016
Application: IHC Sample Tested: Fetal pancreas Species: Human

1:50 on paraffin sections
PDX1 (Red) and DAPI nuclear stain

Immunohistochemistry Human PDX-1/IPF1 Antibody AF2419
Human PDX-1/IPF1 Antibody
By Shijun Yan on 06/29/2016
Application: IHC Sample Tested: Pancreas tissue Species: Mouse