Detection of Human PDZD11 by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PDZD11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5977) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDZD11 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PDZD11 (PDZ domain-containing protein 11; also AIPP1a and PISP) is a 17 kDa cytosolic protein that bears resemblance to members of the MALS/VELIS family of proteins. It is ubiquitously expressed, and appears to target calcium and copper ATPases to basolateral cell membranes. Human PDZD11 is 140 amino acids (aa) in length. It contains but one PDZ domain (aa 47-129) that apparently interacts with the C-terminus of partner proteins. One alternative start site exists that is 32 aa upstream of the standard site. This generates the AIPP1b isoform. Full-length human PDZD11 shares 97% aa identity with mouse PDZD11.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.