Human PGC1 alpha Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB10784S Datasheet / COA / SDS

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FAB10784S-100UG

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Human PGC1 alpha Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PGC1 alpha in direct ELISAs.

Source

Monoclonal Mouse IgG1 Clone # 474915

Purification

Protein A or G purified

Immunogen

E. coli-derived human PGC1Î±
Glu11-Ile280
Accession # Q9UBK2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PGC1 alpha

PGC1 alpha (PPAR-gamma Coactivator 1 alpha; also LEM6) is a 97-120 kDa member of the PGC-1 family of proteins. It is expressed in select cell types, including brown adipocytes, skeletal muscle and hepatocytes. PGC1 alpha participates in both RNA processing and transcriptional coactivation in conjunction with multiple nuclear hormone receptors such as PPAR gamma, RAR and TR. Human PCG1 alpha is 798 amino acids (aa) in length. It contains an LxxLL nuclear receptor binding motif (aa 144-148), one PPAR-gamma interaction domain (aa 293-339), two NLSs and an RNA binding/processing region (aa 566-710). PGC1 alpha activity is regulated by phosphorylation. AMPK is known to phosphorylate Thr178 and Ser539, promoting cotranscriptional activity. Conversely, Akt-mediated phosphorylation at Ser571 is reported to downregulate PGC1 alpha activity. This latter effect is achieved by an initial Ser571 phosphorylation, followed by GCN5 binding and subsequent PCG1 alpha acetylation that promotes PGC-1 alpha dissociation from target gene promoters.

Long Name

Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha

Entrez Gene IDs

10891 (Human); 19017 (Mouse); 83516 (Rat)

Alternate Names

LEM6; Ligand effect modulator 6; ligand effect modulator-6; L-PGC-1alpha; peroxisome proliferative activated receptor, gamma, coactivator 1, alpha; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; PGC1 alpha; PGC1; PGC-1(alpha); PGC1A; PGC-1-alpha; PGC1APGC-1(alpha); PGC1peroxisome proliferative activated receptor, gamma, coactivator 1; PGC-1v; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; PPARgamma coactivator 1alpha; PPAR-gamma coactivator 1-alpha; PPARGC1; PPARGC1A; PPARGC-1-alpha