Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 474915
View all PGC1 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived human PGC1 alpha
Glu11-Ile280
Accession # Q9UBK2

Specificity

Detects human PGC1 alpha in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PGC1 alpha Antibody

Detection of Human PGC1 alpha by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PGC1 alpha Monoclonal Antibody (Catalog # MAB10784) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PGC1 alpha at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

PGC1 alpha in A431 Human Cell Line.

PGC1 alpha was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human PGC1 alpha Monoclonal Antibody (Catalog # MAB10784) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

PGC1 alpha in Human Liver Cancer.

PGC1 alpha was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human PGC1 alpha Monoclonal Antibody (Catalog # MAB10784) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human PGC1 alpha Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 1 review rated 5 using MAB10784 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PGC1 alpha

PGC1 alpha (PPAR-gamma Coactivator 1 alpha; also LEM6) is a 97-120 kDa member of the PGC-1 family of proteins. It is expressed in select cell types, including brown adipocytes, skeletal muscle and hepatocytes. PGC1 alpha participates in both RNA processing and transcriptional coactivation in conjunction with multiple nuclear hormone receptors such as PPAR gamma, RAR and TR. Human PCG1 alpha is 798 amino acids (aa) in length. It contains an LxxLL nuclear receptor binding motif (aa 144-148), one PPAR-gamma interaction domain (aa 293-339), two NLSs and an RNA binding/processing region (aa 566-710). PGC1 alpha activity is regulated by phosphorylation. AMPK is known to phosphorylate Thr178 and Ser539, promoting cotranscriptional activity. Conversely, Akt-mediated phosphorylation at Ser571 is reported to downregulate PGC1 alpha activity. This latter effect is achieved by an initial Ser571 phosphorylation, followed by GCN5 binding and subsequent PCG1 alpha acetylation that promotes PGC-1 alpha dissociation from target gene promoters.

Long Name

Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha

Alternate Names

LEM6, PPARGC1A

Entrez Gene IDs

10891 (Human); 19017 (Mouse); 83516 (Rat)

Gene Symbol

PPARGC1A

UniProt

Q9UBK2 (Human)

Additional PGC1 alpha Products

Product Documents for Human PGC1 alpha Antibody

Certificate of Analysis

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Product Specific Notices for Human PGC1 alpha Antibody

For research use only

Citations for Human PGC1 alpha Antibody

Customer Reviews for Human PGC1 alpha Antibody (1)

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  • Human PGC1 alpha Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver cancer tissue
    Species: Human
    Verified Customer | Posted 06/19/2022
    Human PGC1 alpha Antibody MAB10784

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