Human PGC1 alpha Antibody

Catalog # Availability Size / Price Qty
MAB10784-100
MAB10784-SP
Detection of Human PGC1 alpha  by Western Blot.
3 Images
Product Details
FAQs
Supplemental Products
Reviews (1)

Human PGC1 alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human PGC1 alpha in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 474915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived human PGC1 alpha
Glu11-Ile280
Accession # Q9UBK2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Jurkat human acute T cell leukemia cell line
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of human liver cancer
Immunocytochemistry
8-25 µg/mL
Immersion fixed A431 human epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human PGC1 alpha by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PGC1 alpha Monoclonal Antibody (Catalog # MAB10784) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PGC1 alpha at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry View Larger

PGC1 alpha in A431 Human Cell Line. PGC1 alpha was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human PGC1 alpha Monoclonal Antibody (Catalog # MAB10784) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry View Larger

PGC1 alpha in Human Liver Cancer. PGC1 alpha was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human PGC1 alpha Monoclonal Antibody (Catalog # MAB10784) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGC1 alpha

PGC1 alpha (PPAR-gamma Coactivator 1 alpha; also LEM6) is a 97-120 kDa member of the PGC-1 family of proteins. It is expressed in select cell types, including brown adipocytes, skeletal muscle and hepatocytes. PGC1 alpha participates in both RNA processing and transcriptional coactivation in conjunction with multiple nuclear hormone receptors such as PPAR gamma, RAR and TR. Human PCG1 alpha is 798 amino acids (aa) in length. It contains an LxxLL nuclear receptor binding motif (aa 144-148), one PPAR-gamma interaction domain (aa 293-339), two NLSs and an RNA binding/processing region (aa 566-710). PGC1 alpha activity is regulated by phosphorylation. AMPK is known to phosphorylate Thr178 and Ser539, promoting cotranscriptional activity. Conversely, Akt-mediated phosphorylation at Ser571 is reported to downregulate PGC1 alpha activity. This latter effect is achieved by an initial Ser571 phosphorylation, followed by GCN5 binding and subsequent PCG1 alpha acetylation that promotes PGC-1 alpha dissociation from target gene promoters.

Long Name
Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha
Entrez Gene IDs
10891 (Human); 19017 (Mouse); 83516 (Rat)
Alternate Names
LEM6; Ligand effect modulator 6; ligand effect modulator-6; L-PGC-1alpha; peroxisome proliferative activated receptor, gamma, coactivator 1, alpha; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; PGC1 alpha; PGC1; PGC-1(alpha); PGC1A; PGC-1-alpha; PGC1APGC-1(alpha); PGC1peroxisome proliferative activated receptor, gamma, coactivator 1; PGC-1v; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; PPARgamma coactivator 1alpha; PPAR-gamma coactivator 1-alpha; PPARGC1; PPARGC1A; PPARGC-1-alpha

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human PGC1 alpha Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human PGC1 alpha Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human PGC1 alpha Antibody
By Anonymous on 06/19/2022
Application: IHC Sample Tested: Liver cancer tissue Species: Human