Human Phospho-Bad (S99) Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB7764U Datasheet / COA / SDS

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FAB7764U-100UG

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Human Phospho-Bad (S99) Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Bad when phosphorylated at S99 in direct ELISAs.

Source

Monoclonal Mouse IgG1 Clone # 679431

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human Bad S99 site
Accession # Q92934

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Bad

Bcl-2 antagonist of cell death (Bad) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser75, Ser99, and Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Phosphorylation of Ser75 and Ser99 is also required for the ability of Bad to induce cell cycle arrrest in G1. Human Bad shares 75 % aa sequence identity with mouse and rat Bad.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Entrez Gene IDs

572 (Human); 12015 (Mouse); 64639 (Rat)

Alternate Names

Bad; BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter