|Phospho-Bad (S99) in HEK293 Human Cell Line. Bcl-xL/Bcl-2 Associated Death Promoter phosphorylated at S99 (Phospho-Bad (S99)) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human Phospho-Bad (S99) Monoclonal Antibody (Catalog # MAB7764) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Note that labeling is absent in dividing cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
Bcl-2 antagonist of cell death (Bad) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser75, Ser99, and Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Phosphorylation of Ser75 and Ser99 is also required for the ability of Bad to induce cell cycle arrrest in G1. Human Bad shares 75 % aa sequence identity with mouse and rat Bad.
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