Phospho-Bad (S99) in HEK293 Human Cell Line.
Bcl-xL/Bcl-2 Associated Death Promoter phosphorylated at S99 (Phospho-Bad (S99)) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human Phospho-Bad (S99) Monoclonal Antibody (Catalog # MAB7764) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Note that labeling is absent in dividing cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bcl-2 antagonist of cell death (Bad) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser75, Ser99, and Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Phosphorylation of Ser75 and Ser99 is also required for the ability of Bad to induce cell cycle arrrest in G1. Human Bad shares 75 % aa sequence identity with mouse and rat Bad.
Bcl-xL/Bcl-2 Associated Death Promoter
Entrez Gene IDs:
572 (Human); 12015 (Mouse); 64639 (Rat)
Bad; BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.