Human Phospho-FAK (Y397) Antibody

Catalog # Availability Size / Price Qty
MAB4528
MAB4528-SP
Detection of Human Phospho-FAK (Y397) by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human Phospho-FAK (Y397) Antibody Summary

Species Reactivity
Human
Specificity
Detects human FAK when phosphorylated at Y397.
Source
Monoclonal Rat IgG2A Clone # 820755
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human FAK Y397 site
Accession # Q05397
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-FAK (Y397) antibody by Western Blot. View Larger

Detection of Human Phospho-FAK (Y397) by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAK

Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.

Long Name
Focal adhesion kinase 1
Entrez Gene IDs
5747 (Human); 14083 (Mouse); 25614 (Rat)
Alternate Names
EC 2.7.10; FADK 1; FADK1; FADKFAK-related non-kinase polypeptide; FAK; FAK1FRNK; FAKEC 2.7.10.2; focal adhesion kinase 1; pp125FAK; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; PTK2

Product Datasheets

Citations for Human Phospho-FAK (Y397) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Insight into Cisplatin-Resistance Signaling of W1 Ovarian Cancer Cells Emerges mTOR and HSP27 as Targets for Sensitization Strategies
    Authors: K Wantoch vo, P König, S Henze, M Schlesinge, P Zawierucha, R Januchowsk, G Bendas
    International Journal of Molecular Sciences, 2020;21(23):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin ?4-FAK/Src signaling pathway
    Authors: JH Pak, Q Bashir, IK Kim, SJ Hong, S Maeng, YY Bahk, TS Kim
    Mol. Biochem. Parasitol, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells.
    Authors: Ye F, Zhou F, Nithianantham S, Chandran B, Yu X, Weinberg A, Gao S
    J Virol, 2013;87(11):6326-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human Phospho-FAK (Y397) Antibody

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Human Phospho-FAK (Y397) Antibody
By Anonymous on 04/18/2018
Application: IHC Sample Tested: oct embedded section from mouse pancreati tumor,oct embedded section from mouse pancreatic tumor Species: Mouse

Mouse pancreatic cancer cells were implanted in C57 BL/6 syngeneic host for 1 month
Tumor tissue was fixed in 4% PFA in PBS overnight in cold
Then equilibriated in 30% sucrose in PBS in cold overnight
Embedded in OCT and sectioned at 10um
Stain with antibody for 2h after blocking with Fc recerptor
Counterstained with DAPI