Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.
Human Phospho-FAK (Y397) Antibody
R&D Systems | Catalog # MAB4528
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 820755
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Product Specifications
Immunogen
Phosphopeptide containing the human FAK Y397 site
Accession # Q05397
Accession # Q05397
Specificity
Detects human FAK when phosphorylated at Y397.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Phospho-FAK (Y397) Antibody
Detection of Human Phospho-FAK (Y397) by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-FAK (Y397) by Western Blot
ADAM9 affects auto‐/paracrine MEK/ERK signaling. (A) Western blot of phosphorylation of MEK1/2 (S217/221) and ERK1/2 (T202/204) in AsPC‐1 and MiaPaCa‐2 cells following ADAM9 expression silencing. In AsPC‐1 cells, we observed decreased pFAK (Y397), which was absent in MiaPaCa‐2 cells. Quantitation of pFAK, pMEK1/2, and pERK1/2 in AsPc‐1 and MiaPaCa‐2 cells from three independent experiments. (B) Western blot analysis shows decreased phosphorylation of MEK1/2 (S217/221) and ERK1/2 (T202/204) in HUVEC cells following 30 min of incubation with cancer cell conditioned medium from both AsPC‐1 and MiaPaCa‐2 cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30556643), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Phospho-FAK (Y397) Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate (PV)
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate (PV)
Reviewed Applications
Read 3 reviews rated 4 using MAB4528 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FAK
Long Name
Focal adhesion kinase 1
Alternate Names
FADK1, PTK2
Gene Symbol
PTK2
UniProt
Additional FAK Products
Product Documents for Human Phospho-FAK (Y397) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Phospho-FAK (Y397) Antibody
For research use only
Related Research Areas
Citations for Human Phospho-FAK (Y397) Antibody
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Customer Reviews for Human Phospho-FAK (Y397) Antibody (3)
4 out of 5
3 Customer Ratings
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Application: Western BlotSample Tested: MDA-MB-231 human breast cancer cell lineSpecies: HumanVerified Customer | Posted 05/28/2026No band in MDA-MB-231 cells total protein10 ug of total protein from MDA-MB-231 cells was loaded and antibody used at 1ug/mL as recommended
Bio-Techne ResponseThank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. -
Application: Western BlotSample Tested: HCT-116 human colorectal carcinoma cell lineSpecies: HumanVerified Customer | Posted 05/28/2026No band in HCT116 cells proteinTotal protein of HCT116 cells with recommended dilution
Bio-Techne ResponseThank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
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Application: ImmunohistochemistrySample Tested: oct embedded section from mouse pancreati tumor and oct embedded section from mouse pancreatic tumorSpecies: MouseVerified Customer | Posted 04/18/2018Mouse pancreatic cancer cells were implanted in C57 BL/6 syngeneic host for 1 month Tumor tissue was fixed in 4% PFA in PBS overnight in cold Then equilibriated in 30% sucrose in PBS in cold overnight Embedded in OCT and sectioned at 10um Stain with antibody for 2h after blocking with Fc recerptor Counterstained with DAPI
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
