Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 820755
View all FAK Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human FAK Y397 site
Accession # Q05397

Specificity

Detects human FAK when phosphorylated at Y397.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Phospho-FAK (Y397) Antibody

Detection of Human Phospho-FAK (Y397) antibody by Western Blot.

Detection of Human Phospho-FAK (Y397) by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-FAK (Y397) by Western Blot

Detection of Human Phospho-FAK (Y397) by Western Blot

ADAM9 affects auto‐/paracrine MEK/ERK signaling. (A) Western blot of phosphorylation of MEK1/2 (S217/221) and ERK1/2 (T202/204) in AsPC‐1 and MiaPaCa‐2 cells following ADAM9 expression silencing. In AsPC‐1 cells, we observed decreased pFAK (Y397), which was absent in MiaPaCa‐2 cells. Quantitation of pFAK, pMEK1/2, and pERK1/2 in AsPc‐1 and MiaPaCa‐2 cells from three independent experiments. (B) Western blot analysis shows decreased phosphorylation of MEK1/2 (S217/221) and ERK1/2 (T202/204) in HUVEC cells following 30 min of incubation with cancer cell conditioned medium from both AsPC‐1 and MiaPaCa‐2 cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30556643), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Phospho-FAK (Y397) Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate (PV)

Reviewed Applications

Read 1 review rated 4 using MAB4528 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FAK

Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Entrez Gene IDs

5747 (Human); 14083 (Mouse); 25614 (Rat)

Gene Symbol

PTK2

UniProt

Q05397 (Human)

Additional FAK Products

Product Documents for Human Phospho-FAK (Y397) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-FAK (Y397) Antibody

For research use only

Citations for Human Phospho-FAK (Y397) Antibody

Customer Reviews for Human Phospho-FAK (Y397) Antibody (1)

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  • Human Phospho-FAK (Y397) Antibody
    Name: Mario Shields
    Application: Immunohistochemistry
    Sample Tested: oct embedded section from mouse pancreati tumor and oct embedded section from mouse pancreatic tumor
    Species: Mouse
    Verified Customer | Posted 04/18/2018
    Mouse pancreatic cancer cells were implanted in C57 BL/6 syngeneic host for 1 month Tumor tissue was fixed in 4% PFA in PBS overnight in cold Then equilibriated in 30% sucrose in PBS in cold overnight Embedded in OCT and sectioned at 10um Stain with antibody for 2h after blocking with Fc recerptor Counterstained with DAPI
    Human Phospho-FAK (Y397) Antibody MAB4528

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail