hh Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody AF1094: R&D Systems

Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human MSK1 and MSK2 when phosphorylated at S376 and S360, respectively. The amino acid sequence surrounding S360 of mouse MSK2 is identical to the phosphopeptide antigen.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human MSK1 S376 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line untreated
(-) or treated (+) with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) in the presence or absence of pathway inhibitors SB203580 and PD98059 for 20 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MSK1/MSK2
Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2, also known as RSKB and RPS6KA4, have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.
  • Long Name:
    Mitogen- and Stress-activated Protein Kinase 1 and 2
  • Alternate Names:
    MSK1/MSK2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG Antibody

WB AF008 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information