Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody

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Detection of Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) by Western Blot.
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Product Details
Citations (2)
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Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody Summary

Species Reactivity
Detects human MSK1 and MSK2 when phosphorylated at S376 and S360, respectively. The amino acid sequence surrounding S360 of mouse MSK2 is identical to the phosphopeptide antigen.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide containing human MSK1 S376 site
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) antibody by Western Blot. View Larger

Detection of Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) in the presence or absence of pathway inhibitors SB203580 and PD98059 for 20 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSK1/MSK2

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2, also known as RSKB and RPS6KA4, have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Long Name
Mitogen- and Stress-activated Protein Kinase 1 and 2
Alternate Names
1110069D02Rik; 3110005L17Rik; 6330404E13Rik; MSK1/MSK2

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Citations for Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Generation of a control human induced pluripotent stem cell line using the defective and persistent Sendai virus vector system
    Authors: Z Zhou, S Yoshimatsu, E Qian, M Ishikawa, T Sato, M Ohtaka, M Nakanishi, H Okano
    Stem Cell Research, 2021-09-23;56(0):102549.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs
    Authors: Katarzyna Duda, Lindsey A. Lonowski, Michael Kofoed-Nielsen, Adriana Ibarra, Catherine M. Delay, Qiaohua Kang et al.
    Nucleic Acids Research


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