Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody

Catalog # Availability Size / Price Qty
AF1094
AF1094-SP
Detection of Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody Summary

Species Reactivity
Human
Specificity
Detects human MSK1 and MSK2 when phosphorylated at S376 and S360, respectively. The amino acid sequence surrounding S360 of mouse MSK2 is identical to the phosphopeptide antigen.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human MSK1 S376 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) by Western Blot. View Larger

Detection of Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line untreated
(-) or treated (+) with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) in the presence or absence of pathway inhibitors SB203580 and PD98059 for 20 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSK1/MSK2

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2, also known as RSKB and RPS6KA4, have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Long Name
Mitogen- and Stress-activated Protein Kinase 1 and 2
Alternate Names
MSK1/MSK2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody

There are currently no reviews for this product. Be the first to review Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody and earn rewards!

Have you used Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review