Home / Smad2/3 / Human Phospho-Smad2 (S465/S467)/
Smad3 (S423/S425) Antibody

Human Phospho-Smad2 (S465/S467)/
Smad3 (S423/S425) Antibody

Catalog #: AB3226
3 Citations Datasheet / COA / SDS

Discontinued Product

AB3226 has been discontinued.
View all Smad2/3 products.
Detection of Human Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) by Western Blot.
3 Images
Product Details
Citations (3)
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-Smad2 (S465/S467)/
Smad3 (S423/S425) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Phospho-Smad2/3 when dually phosphorylated at S465/S467 and S423/S425, respectively.
Source
Polyclonal Rabbit Serum
Purification
N/A
Immunogen
Phosphopeptide containing human Smad3 S423/S425 sites
Formulation
Lyophilized from rabbit serum in PBS and Sodium Azide.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:500 dilution
See below
Simple Western
1:50-1:100 dilution
See below
Immunocytochemistry
1:50-1:100 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) antibody by Western Blot. View Larger

Detection of Human Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) by Western Blot. Western blot shows lysates of Mv1Lu mink lung epithelial cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TGF-beta 2 (Catalog # 302-B2) for 24 hours. PVDF membrane was probed with 1:500 dilution of Rabbit Anti-Human Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425)Polyclonal Antibody (Catalog # AB3226), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, loaded at 0.5 mg/mL. A specific band was detected for Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) at approximately 68 kDa (as indicated) using 1:50-1:100 dilution of Rabbit Anti-Human Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) Polyclonal Antibody (Catalog # AB3226). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) antibody in Mv1Lu Mink Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) in Mv1Lu Mink Cell Line. Smad2/3 phosphorylated at S465/S467 and S423/S425 was detected in immersion fixed Mv1Lu mink lung epithelial cell line treated with 10 ng/mL of Recombinant Human TGF-beta 2 (Catalog # 302-B2) using Rabbit Anti-Human Phospho-Smad2 (S465/S467)/ Smad3 (S423/S425) Polyclonal Antibody (Catalog # AB3226) at 1:50-1:100 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute with 250 μL sterile PBS containing 0.02% Sodium Azide.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smad2/3

Smad3 (mothers against DPP homolog 3) is a transcription factor that functions downstream of the transforming growth factor beta receptor (TGF-beta R). Smad3 is phosphorylated in cells stimulated with TGF-beta, complexes with Smad4, and translocates to the nucleus to upregulate gene transcription. Smad3 is critical for signaling fibrosis and wound healing.

Long Name
Mothers Against DPP Homolog 2/3
Alternate Names
hMAD-2; hSMAD2; JV18; JV18-1SMAD, mothers against DPP homolog 2 (Drosophila); MAD homolog 2; Mad protein homolog; Mad, mothers against decapentaplegic homolog 2; MADH2MGC34440; MADR2MGC22139; Mad-related protein 2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila); SMAD, mothers against DPP homolog 2; Smad2; Smad2/3

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Phospho-Smad2 (S465/S467)/
Smad3 (S423/S425) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Loss of canonical Smad4 signaling promotes KRAS driven malignant transformation of human pancreatic duct epithelial cells and metastasis.
    Authors: Leung L, Radulovich N, Zhu C, Wang D, To C, Ibrahimov E, Tsao M
    PLoS ONE, 2013-12-27;8(12):e84366.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  2. Transforming growth factor-beta and oxidative stress mediate tachycardia-induced cellular remodelling in cultured atrial-derived myocytes.
    Authors: Yeh YH, Kuo CT, Chan TH, Chang GJ, Qi XY, Tsai F, Nattel S, Chen WJ
    Cardiovasc. Res., 2011-02-02;91(1):62-70.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  3. Effect of cAMP on TGFbeta1-induced corneal keratocyte-myofibroblast transformation.
    Authors: Xing D, Bonanno JA
    Invest. Ophthalmol. Vis. Sci., 2008-10-20;50(2):626-33.
    Species: Rabbit
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Phospho-Smad2 (S465/S467)/
Smad3 (S423/S425) Antibody

There are currently no reviews for this product. Be the first to review Human Phospho-Smad2 (S465/S467)/
Smad3 (S423/S425) Antibody and earn rewards!

Have you used Human Phospho-Smad2 (S465/S467)/
Smad3 (S423/S425) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review