Key Product Details

Species Reactivity

Human

Applications

Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2175B
View all PILR-alpha Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PILR‑ alpha with a C-terminal 6-His tag 
Gln20-Thr196
Accession # Q9UKJ1

Specificity

Detects human PILR‑ alpha in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human PILR‑ alpha Antibody

PILR-alpha binding to PANP blocked by PILR-alpha Antibody.

PILR-alpha binding to PANP blocked by PILR-alpha Antibody.

In a functional ELISA, Recombinant Human PILR-a (Catalog # 6484-PR) binds to immobilized recombinant human PANP Fc Chimera coated at 5 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PILR-a Monoclonal Antibody (Catalog # MAB64842). At 1-5 µg/mL, this antibody will block 50% of the binding.
Detection of PILR- a R antibody in HEK293 Human Cell Line Transfected with Human PILR-alpha and eGFP antibody by Flow Cytometry.

Detection of PILR- alpha R in HEK293 Human Cell Line Transfected with Human PILR-alpha and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human PILR- a or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human PILR- a Monoclonal Antibody (Catalog # MAB64842) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050).

Applications for Human PILR‑ alpha Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human PILR- alpha and eGFP

Neutralization

In a functional ELISA, 1-5 μg/mL of this antibody will block 50% of the binding of 2 μg/mL Recombinant Human PILR‑ alpha (Catalog # 6484-PR) to immobilized recombinant human PANP Fc Chimera coated at 5 μg/mL (100 µL/well).

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PILR-alpha

PILR-alpha (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by monocytes, macrophages, CD14+CD1a- DC and retinal pigment cells, and is known to bind to CD99 and PANP. PILR-alpha acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta.

Long Name

Paired-Ig-likeType 2 Receptor alpha

Alternate Names

FDF03, PILRA, PILRalpha

Entrez Gene IDs

29992 (Human); 231805 (Mouse); 288568 (Rat)

Gene Symbol

PILRA

UniProt

Q9UKJ1 (Human)

Additional PILR-alpha Products

Product Documents for Human PILR‑ alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PILR‑ alpha Antibody

For research use only

Customer Reviews for Human PILR‑ alpha Antibody

There are currently no reviews for this product. Be the first to review Human PILR‑ alpha Antibody and earn rewards!

Have you used Human PILR‑ alpha Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies