Human PLA2G7/PAF-AH/Lp-PLA2 Antibody

Catalog # Availability Size / Price Qty
MAB5106-100
MAB5106-SP

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Human PLA2G7/PAF‑AH/Lp‑PLA2 ELISA Standard Curve.
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Human PLA2G7/PAF-AH/Lp-PLA2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PLA2G7Phe22-Asn441 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 973010
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PLA2G7/PAF‑AH/Lp‑PLA2
Phe22-Asn441
Accession # Q13093
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human PLA2G7/PAF‑AH/Lp‑PLA2 Monoclonal Antibody (Catalog # MAB51061).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit (Catalog # DPLG70) for a complete optimized ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human PLA2G7/PAF‑AH/Lp‑PLA2 ELISA Standard Curve. Recombinant Human PLA2G7/PAF-AH/Lp-PLA2 protein was serially diluted 2-fold and captured by Mouse Anti-Human PLA2G7/PAF-AH/Lp-PLA2 Monoclonal Antibody (Catalog # MAB51061) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PLA2G7/PAF-AH/Lp-PLA2 Monoclonal Antibody (Catalog # MAB5106) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLA2G7/PAF-AH/Lp-PLA2

Secretory phopholipase A2 is an enzyme that hydrolyses the Sn-2 ester bond of phospholipids, generating free fatty acids and lysophospholipids (1‑3). Most secretory PLA2s are stored in cytoplasmic granules and are released in the extracellular environment on appropriate cell activation. Thus, they are present at higher concentration in the plasma and biologic fluids of patients with systemic inflammatory, autoimmune, or allergic disease, such as acute pancreatitis, rheumatoid arthritis, bronchial asthma, and allergic rhinitis. Also known as Lp-PLA2, PLA2G-VII is a plasma enzyme bound to lipoproteins: 80% bound to LDL, 15%-20% to HDL, and the remainder to VLDL (4-6). It is produced in major by mature macrophages and activated platelets. In contrast to other classical sPLA2s, PLA2G‑VII has poor specificity toward Sn-2 long chain fatty acids, unless heavily oxidized, and undergoes the catalysis of its substrates in the aqueous phase rather than at the interfacial surface of lipids. Thus, it has high specificity for water-soluble phospholipids in plasma including oxidatively‑modified phospholipids and platelet-activating factor (PAF). Because of the latter activity, it is also known as PAF acetylhydrolase (PAF-AH). Lack of human PLA2G‑VII is related to a higher risk for stroke and heart disease.

References
  1. Webb, N. R. (2005) Cur. Opin. Lipid. 16:341.
  2. Triggiani, M. et al. (2005) J. Allergy Clin. Immunol. 116:1000.
  3. Murakami, M. and Kudo, I. (2004) Biol. Pharm. Bull. 27:1158.
  4. Caslake, M. J. and Packard C. J. (2005) Nat. Clin. Prac. Cardiovasc. Med. 2:529.
  5. Karabina, S.-A., and Ninio, E. (2006) Biochim. Biophys. Acta, 1761:1351.
  6. Karasawa, K. (2006) Biochim. Biophys. Acta, 1761:1359.
Long Name
Phospholipase A2 Group VII
Entrez Gene IDs
7941 (Human); 27226 (Mouse); 301265 (Rat)
Alternate Names
2-acetyl-1-alkylglycerophosphocholine esterase; EC 3.1.1; EC 3.1.1.47,1-alkyl-2-acetylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); LDL-PLA2; lipoprotein-associated phospholipase A2; LpPLA2; Lp-PLA2; PAF acetylhydrolase; PAF-AH; PAFAHPAF 2-acylhydrolase; phospholipase A2, group VII (platelet-activating factor acetylhydrolase; PLA2G7; plasma); platelet-activating factor acetylhydrolase

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Isotype Controls

Reconstitution Buffers

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