Human Pleiotrophin/PTN Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Pleiotrophin/PTN in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Pleiotrophin/PTN or recombinant human Midkine is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 851406
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Pleiotrophin/PTN
    Gly33-Asp168
    Accession # P21246
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Pleiotrophin/PTN in PC‑3 Human Cell Line. Pleiotrophin/PTN was detected in immersion fixed PC‑3 human prostate cancer cell line using Rat Anti-Human Pleiotrophin/PTN Monoclonal Antibody (Catalog # MAB252) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pleiotrophin/PTN
Pleiotrophin (PTN), also called heparin-binding growth-associated molecule (HB-GAM), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), or osteoblast-specific factor (OSF-1), is an 18 kDa secreted, strongly heparin-binding, developmentally regulated cytokine (1 ‑ 3). PTN and Midkine share 50% amino acid (aa) sequence identity, share some functions, and constitute a family (1 ‑ 3). The second of two TSP1 domains contains the highest affinity binding site for heparin (4, 5). A 15 kDa form which lacks the C-terminus is mitogenic for glioblastoma cells, while full-length PTN is not (6). PTN is a highly conserved protein; human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequence identity or greater. During development, PTN is involved in development of brain, bone, and organs undergoing branching morphogenesis (3). In the adult, it is induced by PDGF and upregulated in many cancers, hematopoietic stem cells and tissues undergoing remodeling (7 ‑ 10). Cell surface receptors for PTN include Syndecan-3 (which mediates neurite outgrowth) and the receptor tyrosine phosphatase PTPRB, also called RPTP beta / zeta (3, 11 ‑ 13). Heparin binding is necessary for engaging these receptors (7, 8). PTN causes PTPRB dimerization and inactivates its phosphatase activity, which allows increased tyrosine phosphorylation of its substrates (12 ‑ 14). One such substrate is the WNT pathway molecule beta -catenin, allowing crosstalk of PTN with WNTs (12). PTN activation of the receptor ALK (anaplastic lymphoma kinase) is indirect through PTPRB, and mediates mitogenic, transforming and angiogenic activities of PTN (2, 5, 6, 13). Increased expression of PTN is correlated with neuronal development or stresses such as brain ischemia and Parkinson’s disease (2, 3, 7, 8). Both PTN and Midkine have demonstrated bactericidal activity, but only in the absence of heparin (15).
  • References:
    References
    1. Naito, A. et al. (1992) Biochem. Biophys. Res. Commun. 183:701.
    2. Perez-Pinera, P. et al. (2008) Curr. Opin. Hematol. 15:210.
    3. Weng, T. and L. Liu (2010) Respir. Res. 11:80.
    4. Raulo, E. et al. (2005) J. Biol. Chem. 280:41576.
    5. Hamma-Kourbali, Y. et al. (2008) J. Cell. Physiol. 214:250.
    6. Lu, K.V. et al. (2005) J. Biol. Chem. 280:16953.
    7. Yeh, H-J. et al. (1998) J. Neurosci. 18:3699.
    8. Marchionini, D.M. et al. (2007) Brain Res. 1147:77.
    9. Chang, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:10888.
    10. Himburg, H.A. et al. (2010) Nat. Med. 16:475.
    11. Kinnunen, T. et al. (1996) J. Biol. Chem. 271:2243.
    12. Meng, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2603.
    13. Perez-Pinera, P. et al. (2007) J. Biol. Chem. 282:28683.
    14. Fukada, M. et al. (2006) FEBS Lett. 580:4051.
    15. Svensson, S.L. et al. (2010) J. Biol. Chem. 285:16105.
  • Entrez Gene IDs:
    5764 (Human)
  • Alternate Names:
    HARP; HBBM; HB-GAM; HBGF8; HBNF; HBNF1; HBNF-1; Heparin-binding brain mitogen; HNGF-8; NEGF1HBGF-8; neurite growth-promoting factor 1; neurite growth-promoting factor1); OSF-1; Osteoblast-specific factor 1; Pleiotrophin; PTN
Related Research Areas
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Rat HRP-DAB Cell & Tissue Staining Kit

CTS017 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control

Ctrl 6-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B Isotype Control

Ctrl MAB0061R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Primary Antibodies
Description Application Cat# Citations Images  

Human Pleiotrophin/PTN Antibody

ICC MAB252R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Antibody

WB AF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human Pleiotrophin/PTN Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information