Human Pleiotrophin/PTN Antibody (851406R)

R&D Systems | Catalog # MAB252R

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2B Clone # 851406R
View all Pleiotrophin/PTN Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Pleiotrophin/PTN
Gly33-Asp168
Accession # P21246

Specificity

Detects human Pleiotrophin in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human Pleiotrophin/PTN Antibody (851406R)

Pleiotrophin/PTN antibody in PC-3 Human Cell Line by Immunocytochemistry (ICC).

Pleiotrophin/PTN in PC‑3 Human Cell Line.

Pleiotrophin/PTN was detected in immersion fixed PC-3 human prostate cancer cell line using Recombinant Rat Anti-Human Pleiotrophin/PTN Monoclonal Antibody (Catalog # MAB252R) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Pleiotrophin/PTN Antibody (851406R)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Pleiotrophin/PTN Monoclonal Antibody (Catalog # MAB2521 or Catalog # MAB2522 ).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PC‑3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pleiotrophin/PTN

Pleiotrophin (PTN), also called heparin-binding growth-associated molecule (HB-GAM), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), or osteoblast-specific factor (OSF-1), is an 18 kDa secreted, strongly heparin-binding, developmentally regulated cytokine (1-3). PTN and Midkine share 50% amino acid (aa) sequence identity, share some functions, and constitute a family (1-3). The second of two TSP1 domains contains the highest affinity binding site for heparin (4, 5). A 15 kDa form which lacks the C-terminus is mitogenic for glioblastoma cells, while full-length PTN is not (6). PTN is a highly conserved protein; human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequence identity or greater. During development, PTN is involved in development of brain, bone, and organs undergoing branching morphogenesis (3). In the adult, it is induced by PDGF and upregulated in many cancers, hematopoietic stem cells and tissues undergoing remodeling (7-10). Cell surface receptors for PTN include Syndecan-3 (which mediates neurite outgrowth) and the receptor tyrosine phosphatase PTPRB, also called RPTP beta / zeta (3, 11-13). Heparin binding is necessary for engaging these receptors (7, 8). PTN causes PTPRB dimerization and inactivates its phosphatase activity, which allows increased tyrosine phosphorylation of its substrates (12-14). One such substrate is the WNT pathway molecule beta -catenin, allowing crosstalk of PTN with WNTs (12). PTN activation of the receptor ALK (anaplastic lymphoma kinase) is indirect through PTPRB, and mediates mitogenic, transforming and angiogenic activities of PTN (2, 5, 6, 13). Increased expression of PTN is correlated with neuronal development or stresses such as brain ischemia and Parkinson’s disease (2, 3, 7, 8). Both PTN and Midkine have demonstrated bactericidal activity, but only in the absence of heparin (15).

References

  1. Naito, A. et al. (1992) Biochem. Biophys. Res. Commun. 183:701.
  2. Perez-Pinera, P. et al. (2008) Curr. Opin. Hematol. 15:210.
  3. Weng, T. and L. Liu (2010) Respir. Res. 11:80.
  4. Raulo, E. et al. (2005) J. Biol. Chem. 280:41576.
  5. Hamma-Kourbali, Y. et al. (2008) J. Cell. Physiol. 214:250.
  6. Lu, K.V. et al. (2005) J. Biol. Chem. 280:16953.
  7. Yeh, H-J. et al. (1998) J. Neurosci. 18:3699.
  8. Marchionini, D.M. et al. (2007) Brain Res. 1147:77.
  9. Chang, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:10888.
  10. Himburg, H.A. et al. (2010) Nat. Med. 16:475.
  11. Kinnunen, T. et al. (1996) J. Biol. Chem. 271:2243.
  12. Meng, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2603.
  13. Perez-Pinera, P. et al. (2007) J. Biol. Chem. 282:28683.
  14. Fukada, M. et al. (2006) FEBS Lett. 580:4051.
  15. Svensson, S.L. et al. (2010) J. Biol. Chem. 285:16105.

Alternate Names

HARP, HBNF, HNGF-8, OSF-1, PTN

Entrez Gene IDs

5764 (Human)

Gene Symbol

PTN

UniProt

P21246

Additional Pleiotrophin/PTN Products

Product Documents for Human Pleiotrophin/PTN Antibody (851406R)

Certificate of Analysis

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Product Specific Notices for Human Pleiotrophin/PTN Antibody (851406R)

For research use only

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Protocols

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