Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 851407
View all Pleiotrophin/PTN Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Pleiotrophin/PTN
Gly33-Asp168
Accession # P21246

Specificity

Detects human Pleotrophin/PTN in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human Pleiotrophin/PTN Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Human Pleiotrophin/PTN Monoclonal Antibody (Catalog # MAB252R).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage


Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Pleiotrophin/PTN

Pleiotrophin (PTN), also called heparin-binding growth-associated molecule (HB-GAM), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), or osteoblast-specific factor (OSF-1), is an 18 kDa secreted, strongly heparin-binding, developmentally regulated cytokine (1 ‑ 3). PTN and Midkine share 50% amino acid (aa) sequence identity, share some functions, and constitute a family (1 ‑ 3). The second of two TSP1 domains contains the highest affinity binding site for heparin (4, 5). A 15 kDa form which lacks the C-terminus is mitogenic for glioblastoma cells, while full-length PTN is not (6). PTN is a highly conserved protein; human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequence identity or greater. During development, PTN is involved in development of brain, bone, and organs undergoing branching morphogenesis (3). In the adult, it is induced by PDGF and upregulated in many cancers, hematopoietic stem cells and tissues undergoing remodeling (7 ‑ 10). Cell surface receptors for PTN include Syndecan-3 (which mediates neurite outgrowth) and the receptor tyrosine phosphatase PTPRB, also called RPTP beta / zeta (3, 11 ‑ 13). Heparin binding is necessary for engaging these receptors (7, 8). PTN causes PTPRB dimerization and inactivates its phosphatase activity, which allows increased tyrosine phosphorylation of its substrates (12 ‑ 14). One such substrate is the WNT pathway molecule beta -catenin, allowing crosstalk of PTN with WNTs (12). PTN activation of the receptor ALK (anaplastic lymphoma kinase) is indirect through PTPRB, and mediates mitogenic, transforming and angiogenic activities of PTN (2, 5, 6, 13). Increased expression of PTN is correlated with neuronal development or stresses such as brain ischemia and Parkinson’s disease (2, 3, 7, 8). Both PTN and Midkine have demonstrated bactericidal activity, but only in the absence of heparin (15).

References

References
  1. Naito, A. et al. (1992) Biochem. Biophys. Res. Commun. 183:701.
  2. Perez-Pinera, P. et al. (2008) Curr. Opin. Hematol. 15:210.
  3. Weng, T. and L. Liu (2010) Respir. Res. 11:80.
  4. Raulo, E. et al. (2005) J. Biol. Chem. 280:41576.
  5. Hamma-Kourbali, Y. et al. (2008) J. Cell. Physiol. 214:250.
  6. Lu, K.V. et al. (2005) J. Biol. Chem. 280:16953.
  7. Yeh, H-J. et al. (1998) J. Neurosci. 18:3699.
  8. Marchionini, D.M. et al. (2007) Brain Res. 1147:77.
  9. Chang, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:10888.
  10. Himburg, H.A. et al. (2010) Nat. Med. 16:475.
  11. Kinnunen, T. et al. (1996) J. Biol. Chem. 271:2243.
  12. Meng, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2603.
  13. Perez-Pinera, P. et al. (2007) J. Biol. Chem. 282:28683.
  14. Fukada, M. et al. (2006) FEBS Lett. 580:4051.
  15. Svensson, S.L. et al. (2010) J. Biol. Chem. 285:16105.

Alternate Names

HARP, HBNF, HNGF-8, OSF-1, PTN

Entrez Gene IDs

5764 (Human)

Gene Symbol

PTN

UniProt

P21246

Additional Pleiotrophin/PTN Products

Product Documents for Human Pleiotrophin/PTN Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Pleiotrophin/PTN Antibody

For research use only

Customer Reviews for Human Pleiotrophin/PTN Antibody

There are currently no reviews for this product. Be the first to review Human Pleiotrophin/PTN Antibody and earn rewards!

Have you used Human Pleiotrophin/PTN Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies