Detection of Human Plexin A4 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Plexin A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5856) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A4 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin A4
Plexin A4 is a 220‑230 kDa member of the plexin A subfamily, plexin family of proteins (1). It is found on sensory, autonomic and motor neurons and oligodendrocytes, plus T cells and dendritic cells (1‑8). Mature human Plexin A4 is an 1871 amino acid (aa) type I transmembrane glycoprotein with a 23 aa signal sequence, a 1214 aa extracellular domain (ECD), and a 636 aa cytoplasmic region. The ECD contains one Sema-domain (aa 51‑482), three PSI domains (aa 509‑856) and four IPT regions (aa 858‑1230) that contain a phosphoserine at aa 946 (1). Of three isoform variants, one shows a 65 aa substitution for aa 458‑1894, a second shows an 80 aa substitution for aa 1292‑1894, and a third shows the just mentioned 80 aa substitution coupled to a 14 aa substitution for aa 1‑535 (9). The human Plexin A4 ECD shares 97% aa identity with mouse, equine, canine, and bovine Plexin A4. Full‑length Plexin A4 also shares 67% aa identity with the most related family member, Plexin A2. Plexin A4 regulates cell migration, activation and axon guidance via repulsion (1‑5). It serves as a receptor for transmembrane semaphorins, Sema6A and 6B, and as a coreceptor with neuropilin-1 for the secreted semaphorin, Sema3A (1‑8). During development, it plays a role in nerve migration and midline crossing and down‑regulates dendrite formation (2‑8). It is often co‑expressed with Plexin A3, which can also engage class 6 semaphorins but prefers Sema3F/ neuropilin‑2 to Sema3A/neuropilin-1 (3, 8). Thus, Plexins A3 and A4 are redundant in some functions, but unique in others. In T cells, Plexin A4 engages Sema3A and negatively regulates TCR signals (6).
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Protein Accession # NP_861440, EAW83796, EAL24077.
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