Human Polycystin-1/PKD1 Antibody

Catalog # Availability Size / Price Qty
Polycystin‑1/PKD1 in Human HUVEC.
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Product Details
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Human Polycystin-1/PKD1 Antibody Summary

Species Reactivity
Detects human Polycystin-1/PKD1 in direct ELISAs.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human Polycystin-1/PKD1
Accession # P98161
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Polycystin-1/PKD1 antibody in Human HUVEC by Immunocytochemistry (ICC). View Larger

Polycystin‑1/PKD1 in Human HUVEC. Polycystin‑1/PKD1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human Polycystin‑1/PKD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6270) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membranes, cytoplasm and endoplasmic reticula. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Polycystin-1/PKD1

Polycystin-1 (also PKD1) is a 500-550 kDa member of the polycystin family of proteins. It is expressed in renal tubule primary cilia, and the membrane region that forms adherens junctions. Polycystin-1 binds to polycystin-2, promoting its insertion into the cell membrane, and regulating its calcium channel activity. In conjunction with polycystin-2, it detects fluid flow and converts this information into calcium signals. It also exists in the ER, where it negatively modulates polycystin-2 mediated calcium release. Mature human polycystin-1 is a 4280 amino acid (aa), 11 transmembrane glycoprotein. Its N-terminal extracellular region (aa 24-3074) is highly modular, and contains a C-type lecin domain, multiple Leu-rich repeats and PKD domains, one GPS region and a PLAT, REJ, WSC and LDLR domain. The cytoplasmic C-terminus (aa 4107-4303) contains a polycystin-2 coiled-coil interaction domain (aa 4220-4251). A percentage of polycystin-1 undergoes autoproteolysis after Leu3048, generating a presumed N-terminal fragment of ~ 350-400 kDa, and a membrane-bound C-terminal fragment of 150 kDa. Over aa 4141-4303, human polycystin-1 shares 71% aa identity with mouse polycystin-1.

Entrez Gene IDs
5310 (Human); 18763 (Mouse)
Alternate Names
Autosomal dominant polycystic kidney disease 1 protein; PBP; Pc-1; PKD1; polycystic kidney disease 1 (autosomal dominant); polycystic kidney disease-associated protein; polycystin 1; Polycystin1; Polycystin-1; transient receptor potential cation channel, subfamily P, member 1; TRPP1

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