Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PPP2R4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PPP2R4
Glu14-Asn323
Accession # Q15257

Specificity

Detects human PPP2R4 in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PPP2R4 Antibody

Detection of Human PPP2R4 antibody by Western Blot.

Detection of Human PPP2R4 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, U266 human myeloma cell line, and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PPP2R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5929) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PPP2R4 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PPP2R4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line, U266 human myeloma cell line, and HDLM‑2 human Hodgkin's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PPP2R4

PPP2R4, also known as serine/threonine protein phosphatase 2A regulatory subunit B’, PR53 isoform, and PTPA, is a widely expressed member of the PTPA-type peptidyl prolyl isomerase family. Human PPP2R4 is 358 amino acids (aa) in length. Three splicing variants produce four isoforms for PPP2R4, and isoform 2 is the standard protein. Isoform 1, isoform 3 and isoform 4 have deletions corresponding to aa 73-107, 45-108, and 73-149 in isoform 2, respectively. Human PPP2R4 is 87% aa identical to mouse and rabbit PPP2R4. PPP2R4 reversibly stimulates the variable phosphotyrosyl phosphatase activity of the PP2A core heterodimer in the presence of ATP and Mg2+. It has been shown that p53 downregulates the expression of PPP2R4.

Long Name

Serine/threonine Protein Phosphatase 2A Regulatory Subunit B

Alternate Names

PTPA

Entrez Gene IDs

5524 (Human); 110854 (Mouse)

Gene Symbol

PTPA

UniProt

Q15257

Additional PPP2R4 Products

Product Documents for Human PPP2R4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PPP2R4 Antibody

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

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