Human Pro-Collagen I alpha 1 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY6220-05
Ancillary Products Available
Human Collagen I alpha 1 ELISA Standard Curve
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Product Details
Citations (24)
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Human Pro-Collagen I alpha 1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Collagen I alpha 1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human Collagen I alpha 1 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I (alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

Entrez Gene IDs:
1277 (Human)
Alternate Names:
Alpha-1 type I collagen; COL1A1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; Collagen Type I Alpha 1 Chain; collagen, type I, alpha 1; Collagen1; Collagen-1; EDSARTH1; OI4; pro-alpha-1 collagen type 1; Type I Procollagen Alpha 1 Chain

Citations for Human Pro-Collagen I alpha 1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. &alphaAzithromycin has enhanced effects on lung fibroblasts from idiopathic pulmonary fibrosis (IPF) patients compared to controls
    Authors: K Krempaska, S Barnowski, J Gavini, N Hobi, S Ebener, C Simillion, A Stokes, R Schliep, L Knudsen, TK Geiser, M Funke-Cham
    Respir. Res., 2020;21(1):25.
    Species: Human
    Sample Types: Cell Culture Superantes
  2. Antiphotoaging effect of boiled abalone residual peptide ATPGDEG on UVB-induced keratinocyte HaCaT cells
    Authors: J Chen, P Liang, Z Xiao, MF Chen, F Gong, C Li, C Zhou, P Hong, WK Jung, ZJ Qian
    Food Nutr Res, 2019;63(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Attenuation of diabetic cardiomyopathy by relying on kirenol to suppress inflammation in a diabetic rat model
    Authors: B Wu, XY Huang, L Li, XH Fan, PC Li, CQ Huang, J Xiao, R Gui, S Wang
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  4. Hydrangenol Isolated from the Leaves of Hydrangea serrata Attenuates Wrinkle Formation and Repairs Skin Moisture in UVB-Irradiated Hairless Mice
    Authors: DB Myung, HS Han, JS Shin, JY Park, HJ Hwang, HJ Kim, HS Ahn, SH Lee, KT Lee
    Nutrients, 2019;11(10):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Primary Human Fibroblasts in Culture Switch to a Myofibroblast-Like Phenotype Independently of TGF Beta
    Authors: U Baranyi, B Winter, A Gugerell, B Hegedus, C Brostjan, G Laufer, B Messner
    Cells, 2019;8(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Probucol ameliorates EMT and lung fibrosis through restoration of SIRT3 expression
    Authors: HX Zhang, YN Li, XL Wang, CL Ye, XY Zhu, HP Li, T Yang, YJ Liu
    Pulm Pharmacol Ther, 2019;0(0):101803.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. WNT3a and WNT5a Transported by Exosomes Activate WNT Signaling Pathways in Human Cardiac Fibroblasts
    Authors: E Dzia?o, M Rudnik, RI Koning, M Czepiel, K Tkacz, M Baj-Krzywo, O Distler, M Siedlar, G Kania, P B?yszczuk
    Int J Mol Sci, 2019;20(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. microRNA-29a inhibits cardiac fibrosis in Sprague-Dawley rats by downregulating the expression of DNMT3A
    Authors: RH Qin, H Tao, SH Ni, P Shi, C Dai, KH Shi
    Anatol J Cardiol, 2018;20(4):198-205.
    Species: Rat
    Sample Types: Serum
  9. Protective Effects of LSGYGP from Fish Skin Gelatin Hydrolysates on UVB-Induced MEFs by Regulation of Oxidative Stress and Matrix Metalloproteinase Activity
    Authors: Q Ma, Q Liu, L Yuan, Y Zhuang
    Nutrients, 2018;10(4):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. The Potential Role of Bile Acids in Acquired Laryngotracheal Stenosis
    Authors: A Aldhahrani, J Powell, S Ladak, M Ali, S Ali, B Verdon, J Pearson, C Ward
    Laryngoscope, 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  11. Gender-independent efficacy of mesenchymal stem cell therapy in sex hormone-deficient bone loss via immunosuppression and resident stem cell recovery
    Authors: BD Sui, J Chen, XY Zhang, T He, P Zhao, CX Zheng, M Li, CH Hu, Y Jin
    Exp. Mol. Med., 2018;50(12):166.
    Species: Human
    Sample Types: Serum
  12. Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts
    Authors: E Raschi, CB Chighizola, L Cesana, D Privitera, F Ingegnoli, C Mastaglio, PL Meroni, MO Borghi
    Arthritis Res. Ther., 2018;20(1):187.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking
    Authors: Y Kondo, J Fu, H Wang, C Hoover, JM McDaniel, R Steet, D Patra, J Song, L Pollard, S Cathey, T Yago, G Wiley, S Macwana, J Guthridge, S McGee, S Li, C Griffin, K Furukawa, JA James, C Ruan, RP McEver, KJ Wierenga, PM Gaffney, L Xia
    JCI Insight, 2018;3(14):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression
    Authors: AR Han, TG Lim, YR Song, M Jang, YK Rhee, HD Hong, MH Kim, HJ Kim, CW Cho
    Int J Mol Sci, 2018;19(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Anti-Tumour Necrosis Factor Therapy for Dupuytren's Disease: A Randomised Dose Response Proof of Concept Phase 2a Clinical Trial
    Authors: J Nanchahal, C Ball, D Davidson, L Williams, W Sones, FE McCann, M Cabrita, J Swettenham, NJ Cahoon, B Copsey, E Anne Franc, PC Taylor, J Black, VS Barber, S Dutton, M Feldmann, SE Lamb
    EBioMedicine, 2018;0(0):.
    Species: Human
    Sample Types: Serum
  16. Asiaticoside hinders the invasive growth of keloid fibroblasts through inhibition of the GDF-9/MAPK/Smad pathway
    Authors: X Wu, D Bian, Y Dou, Z Gong, Q Tan, Y Xia, Y Dai
    J. Biochem. Mol. Toxicol, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Dynamin-Related Protein 1 Inhibition Attenuates Cardiovascular Calcification in the Presence of Oxidative Stress
    Authors: M Rogers, N Maldonado-, JD Hutcheson, C Goettsch, S Goto, I Yamada, T Faits, H Sesaki, M Aikawa, E Aikawa
    Circ. Res., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders
    Authors: A Beierfu beta, H Dietrich, C Kremser, M Hunjadi, A Ritsch, T Rülicke, C Thomé, DS Mern
    PLoS ONE, 2017;12(11):e0187564.
    Species: Rabbit
    Sample Types: Tissue Homogenates
  19. Differential Proteomics Identifies Reticulocalbin-3 as a Novel Negative Mediator of Collagen Production in Human Cardiac Fibroblasts
    Authors: E Martínez-M, J Ibarrola, A Fernández-, E Santamaria, J Fernández-, P Rossignol, F Jaisser, N López-Andr
    Sci Rep, 2017;7(1):12192.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
    JCI Insight, 2016;1(20):e90954.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment
    Cancer Res., 2016;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
  22. Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1? or TNF-? Release from Human Hepatic Stellate Cells
    Authors: S Robert, T Gicquel, A Bodin, V Lagente, E Boichot
    PLoS ONE, 2016;11(4):e0153118.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. The atherogenic Scarb1 null mouse model shows a high bone mass phenotype.
    Authors: Martineau C, Martin-Falstrault L, Brissette L, Moreau R
    Am J Physiol Endocrinol Metab, 2014;306(1):E48-57.
    Species: Mouse
    Sample Types: Plasma
  24. Angiotensin-(1-7) and angiotensin induce the transdifferentiation of human endometrial epithelial cells in vitro.
    Authors: Shan T, Zhang L, Zhao C, Chen W, Zhang Y, Li G
    Mol Med Rep, 2014;9(6):2180-6.
    Species: Human
    Sample Types: Cell Culture Supernates

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