Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I (alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.
Human Pro-Collagen I alpha 1 DuoSet ELISA
R&D Systems | Catalog # DY6220-05
Key Product Details
Assay Type
Assay Range
Sample Type
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human Pro-Collagen I alpha 1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Pro-Collagen I alpha 1 DuoSet ELISA
Product Specifications
Assay Format
Sample Volume Required
Detection Method
Conjugate
Specificity
Label
Scientific Data Images for Human Pro-Collagen I alpha 1 DuoSet ELISA
Human Collagen I alpha 1 ELISA Standard Curve
Kit Contents for Human Pro-Collagen I alpha 1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Preparation and Storage
Shipping
Stability & Storage
Background: Collagen I alpha 1
Alternate Names
Gene Symbol
Additional Collagen I alpha 1 Products
Product Documents for Human Pro-Collagen I alpha 1 DuoSet ELISA
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Pro-Collagen I alpha 1 DuoSet ELISA
For research use only
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Citations for Human Pro-Collagen I alpha 1 DuoSet ELISA
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Customer Images
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Sample Tested: Cell culture supernatant and HDFn culture supernatantVerified Customer | Posted 06/13/2023Standard curve obtained with Human Pro-Collagen I alpha 1 DuoSet ELISA DY6220
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Sample Tested: corneal stromal stem cellVerified Customer | Posted 03/01/2022
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Sample Tested: Cell culture supernatantVerified Customer | Posted 12/09/2021Dermal fibroblast cell culture supernatant following stimulation with 2ng/mL TGFb1
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Sample Tested: SerumVerified Customer | Posted 11/03/2021
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Sample Tested: NF03-02 human fibroblastVerified Customer | Posted 09/13/2021
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Sample Tested: condition mediumVerified Customer | Posted 08/19/2021
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Sample Tested: Cell Culture MediaVerified Customer | Posted 08/06/2021
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Sample Tested: Cell culture supernatantVerified Customer | Posted 04/29/2021using supernatants from primary lung fibroblasts
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Sample Tested: Cell LysatesVerified Customer | Posted 12/01/2020
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Sample Tested: Cell culture supernatant and Tissue Culture MediaVerified Customer | Posted 11/21/2018
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Sample Tested: HASMC human aortic smooth muscle cellsVerified Customer | Posted 10/08/2018
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Sample Tested: SerumVerified Customer | Posted 08/29/2018
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Sample Tested: Human cell conditioned medium and stellate cellsVerified Customer | Posted 08/23/2018
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Sample Tested: Liver cellsVerified Customer | Posted 05/31/2018
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Sample Tested: Bone ExtractsVerified Customer | Posted 09/08/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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