Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit

R&D Systems | Catalog # DLBP00

R&D Systems
Discontinued Product
DLBP00 has been discontinued. View all IL-1 beta/IL-1F2 products.

Key Product Details

Assay Length

3 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL)

Sensitivity

7.2 pg/mL (Cell Culture Supernates)
8.9 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Assay Range

23.4-1500 pg/mL (Cell Culture Supernates)
31.2-2000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
View all IL-1 beta/IL-1F2 ELISA Kits »

Product Summary for Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit

The Quantikine Human Pro-IL-1 beta is a sandwich enzyme-linked immunoassay. The capture antibody coated to the microplate is specific for the pro-piece of the molecule (first 116 residues), and the detection antibody is specific for mature IL-1 beta. Thus, the assay does not detect either the pro-piece alone or the mature form alone; it is specific instead for the intact pro-IL-1 beta.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human pro-IL-1 beta

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 85.5 240 432 85.1 248 457
Standard Deviation 4.7 11.5 20.8 6.2 14.0 28.3
CV% 5.5 4.8 4.8 7.3 5.6 6.2

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 126 374 670 121 342 626
Standard Deviation 6.4 15.7 34.5 8.8 17.9 34.9
CV% 5.1 4.2 5.1 7.3 5.2 5.6

Recovery for Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit

The recovery of pro-IL-1 beta spiked to three different levels throughout the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 108 99-115
EDTA Plasma (n=5) 103 95-108
Heparin Plasma (n=5) 101 92-109
Serum (n=5) 102 97-108

Linearity

To assess the linearity of the assay, five samples spiked with high concentrations of pro-IL-1 beta were diluted in the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Pro-IL-1 beta/IL-1F2 ELISA Linearity

Scientific Data Images for Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit

Human Pro-IL-1 beta/IL-1F2 ELISA Cell Culture Supernate Standard Curve

Human Pro-IL-1 beta/IL-1F2 ELISA Cell Culture Supernate Standard Curve

Human Pro-IL-1 beta/IL-1F2 ELISA Serum/Plasma Standard Curve

Human Pro-IL-1 beta/IL-1F2 ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

Additional IL-1 beta/IL-1F2 Products

Product Documents for Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit

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    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 03/31/2017
    Highly Recommended.

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Protocols

View specific protocols for Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit (DLBP00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 200 µL Standard, Control, or Sample
  4.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1.5 hours.
  5.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.
    6. 100 µL Antiserum
  6.   Add 100 µL of Antiserum to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
  7.   Aspirate and wash 3 times.
    8. 100 µL Conjugate
  8.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
  9.   Aspirate and wash 3 times.
    10. 200 µL Substrate Solution
  10. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
    11. 50 µL Stop Solution
  11. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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