Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit

(14 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL)
  • Sensitivity
    8.9 pg/mL
  • Assay Range
    23.4 - 1,500 pg/mL (Cell Culture Supernates), 31.2 - 2,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human pro-IL-1 beta
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human Pro-IL-1 beta is a sandwich enzyme-linked immunoassay. The capture antibody coated to the microplate is specific for the pro-piece of the molecule (first 116 residues), and the detection antibody is specific for mature IL-1 beta. Thus, the assay does not detect either the pro-piece alone or the mature form alone; it is specific instead for the intact pro-IL-1 beta.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation4.711.520.86.21428.3

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation6.415.734.58.817.934.9


The recovery of pro-IL-1 beta spiked to three different levels throughout the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 108 99-115
EDTA Plasma (n=5) 103 95-108
Heparin Plasma (n=5) 101 92-109
Serum (n=5) 102 97-108
To assess the linearity of the assay, five samples spiked with high concentrations of pro-IL-1 beta were diluted in the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 beta/IL-1F2
IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.
    • Long Name
      Interleukin 1 beta
    • Entrez Gene IDs
      3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig);
    • Alternate Names
      catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, Control, or Sample
    6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1.5 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 100 µL Antiserum
    9.   Add 100 µL of Antiserum to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
    10.   Aspirate and wash 3 times.

    11. 100 µL Conjugate
    12.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
    13.   Aspirate and wash 3 times.

    14. 200 µL Substrate Solution
    15. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

    16. 50 µL Stop Solution
    17. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 14
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    Sample Type
    1. Extracorporeal photopheresis promotes IL-1beta production.
      Authors: Yakut E, Jakobs C, Peric A, Michel G, Baal N, Bein G, Brune B, Hornung V, Hackstein H
      J Immunol, 2015;194(6):2569-77.
      Species: Human
      Sample Type: cell supernates
    2. Complement C5a potentiates uric acid crystal-induced IL-1beta production.
      Authors: An L, Mehta P, Xu L, Turman S, Reimer T, Naiman B, Connor J, Sanjuan M, Kolbeck R, Fung M
      Eur J Immunol, 2014;44(12):3669-79.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils.
      Authors: Haddad L, Sirois M
      PLoS ONE, 2014;9(2):e88980.
      Species: Human
      Sample Type: Cell Lysates
    4. Self double-stranded (ds)DNA induces IL-1beta production from human monocytes by activating NLRP3 inflammasome in the presence of anti-dsDNA antibodies.
      Authors: Shin M, Kang Y, Lee N, Wahl E, Kim S, Kang K, Lazova R, Kang I
      J Immunol, 2013;190(4):1407-15.
      Species: Human
      Sample Type: cell culture supernate
    5. Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling.
      Authors: Beynon V, Quintana FJ, Weiner HL
      PLoS ONE, 2012;7(6):e39576.
      Species: Human
      Sample Type: Serum
    6. Yersinia pseudotuberculosis disrupts intestinal barrier integrity through hematopoietic TLR-2 signaling.
      Authors: Jung C, Meinzer U, Montcuquet N, Thachil E, Chateau D, Thiebaut R, Roy M, Alnabhani Z, Berrebi D, Dussaillant M, Pedruzzi E, Thenet S, Cerf-Bensussan N, Hugot JP, Barreau F
      J. Clin. Invest., 2012;122(6):2239-51.
      Species: Human
      Sample Type: Cell Lysates
    7. Reactive oxygen species-independent activation of the IL-1beta inflammasome in cells from patients with chronic granulomatous disease.
      Authors: van de Veerdonk FL, Smeekens SP, Joosten LA, Kullberg BJ, Dinarello CA, Van der Meer JW, Netea MG
      Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3030-3.
      Species: Human
      Sample Type: Cell Lysates
    8. Inflammatory arthritis in caspase 1 gene-deficient mice: contribution of proteinase 3 to caspase 1-independent production of bioactive interleukin-1beta.
      Authors: Joosten LA, Netea MG, Fantuzzi G, Koenders MI, Helsen MM, Sparrer H, Pham CT, Van der Meer JW, Dinarello CA, van den Berg WB
      Arthritis Rheum., 2009;60(12):3651-62.
      Species: Human
      Sample Type: recombinant pro-IL-1 beta
    9. Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity.
      Authors: Li J, Yin HL, Yuan J
      J. Cell Biol., 2008;181(2):321-33.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. HMG-CoA reductase inhibition induces IL-1beta release through Rac1/PI3K/PKB-dependent caspase-1 activation.
      Authors: Kuijk LM, Beekman JM, Koster J, Waterham HR, Frenkel J, Coffer PJ
      Blood, 2008;112(9):3563-73.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Abundant secretion of bioactive interleukin-1beta by human macrophages induced by Actinobacillus actinomycetemcomitans leukotoxin.
      Authors: Kelk P, Claesson R, Hanstrom L, Lerner UH, Kalfas S, Johansson A
      Infect. Immun., 2005;73(1):453-8.
      Species: Human
      Sample Type: Cell Lysates
    12. Concentrations of macrophage inflammatory proteins MIP-1alpha and MIP-1beta and interleukin 8 (il-8) in lyme borreliosis.
      Authors: Grygorczuk S, Pancewicz S, Zajkowska J, Kondrusik M, Rwierzbinska R, Hermanowska-Szpakowicz T
      Infection, 2004;32(6):350-5.
      Species: Human
      Sample Type: CSF
    13. Activated platelets mediate inflammatory signaling by regulated interleukin 1beta synthesis.
      Authors: Lindemann S, Tolley ND, Dixon DA, McIntyre TM, Prescott SM, Zimmerman GA, Weyrich AS
      J. Cell Biol., 2001;154(3):485-90.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia.
      Authors: Yates SL, Burgess LH, Kocsis-Angle J, Antal JM, Dority MD, Embury PB, Piotrkowski AM, Brunden KR
      J. Neurochem., 2000;74(3):1017-25.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 617 Human Pro-IL-1 beta

    Ctrl QC145
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    Supplemental ELISA Products
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    Quantikine Wash Buffer 1

    ELISA WA126 5
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