Home / MMP-1 / Human Pro MMP-1 Antibody

Human Pro MMP-1 Antibody

Catalog #: MAB900
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB900 has been discontinued.
View all MMP-1 products.
Product Details
Citations (1)
FAQs
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pro MMP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pro MMP-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) active MMP-1, rhMMP-2, rhMMP-3, rhMMP-8, or rhMMP-9 is observed.
Source
Monoclonal Mouse IgG1 Clone # 36660
Purification
Protein A or G purified from ascites
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-1
Phe20-Asn469 (predicted)
Accession # P03956
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑1 Western Blot Standard (Catalog # WBC024)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑1 (Catalog # 901-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-1

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, alpha -1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGF-BP3, IGF-BP5, pro MMP-2 and pro MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

References
  1. Cawston, T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.
Long Name
Matrix Metalloproteinase 1
Entrez Gene IDs
4312 (Human); 83995 (Mouse)
Alternate Names
CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC 3.4.24.7; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP1; MMP-1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human Pro MMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
    Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2007-05-01;178(9):5957-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Pro MMP-1 Antibody

There are currently no reviews for this product. Be the first to review Human Pro MMP-1 Antibody and earn rewards!

Have you used Human Pro MMP-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review