Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antibody Summary
Accession # Q9P0J1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Pyruvate Dehydrogenase Phosphatase/PDP1 by Western Blot. Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pyruvate Dehydrogenase Phosphatase/PDP1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pyruvate Dehydrogenase Phosphatase/PDP1
PPM2C (Protein phosphatase mitochondrial 2C; also PDP1) is a 52-54 kDa member of the PP2C family of enzymes. It is expressed in skeletal muscle and adipocytes, and represents one of two subunits that comprise PDP (pyruvate dehydrogenase phosphatase). PPMC2 is found within the mitochondrial matrix space where, as part of PDP, it dephosphorylates the E1 component of the pyruvate dehydrogenase complex, resulting in its activation, and the downstream generation of acetyl-CoA and NADH from pyruvate. Human PPM2C is 537 amino acids (aa) in length. It contains a mitochondrion targeting sequence (aa 1-71), followed by a mature region that possesses a catalytic domain between aa 109-440. There are three potential isoform variants. Two contain alternative start sites 25 and 59 aa upstream of the standard start site, respectively. A third shows a 12 aa substitution for aa 105-116. Over aa 72-537, human PPM2C shares 99% aa identity with mouse PPM2C.
Citation for Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Murine Cytomegalovirus Spreads by Dendritic Cell Recirculation
Authors: HE Farrell, K Bruce, C Lawler, M Oliveira, R Cardin, N Davis-Poyn, PG Stevenson
Sample Types: Whole Tissue
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antibody
There are currently no reviews for this product. Be the first to review Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antibody and earn rewards!
Have you used Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image