Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antibody

Catalog #: AF7260
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7260
AF7260-SP

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Detection of Human Pyruvate Dehydrogenase Phosphatase/PDP1 by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pyruvate Dehydrogenase Phosphatase/PDP1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Pyruvate Dehydrogenase Phosphatase/PDP1
Ala72-Glu537
Accession # Q9P0J1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Pyruvate Dehydrogenase Phosphatase/PDP1 by Western Blot. Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pyruvate Dehydrogenase Phosphatase/PDP1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pyruvate Dehydrogenase Phosphatase/PDP1

PPM2C (Protein phosphatase mitochondrial 2C; also PDP1) is a 52-54 kDa member of the PP2C family of enzymes. It is expressed in skeletal muscle and adipocytes, and represents one of two subunits that comprise PDP (pyruvate dehydrogenase phosphatase). PPMC2 is found within the mitochondrial matrix space where, as part of PDP, it dephosphorylates the E1 component of the pyruvate dehydrogenase complex, resulting in its activation, and the downstream generation of acetyl-CoA and NADH from pyruvate. Human PPM2C is 537 amino acids (aa) in length. It contains a mitochondrion targeting sequence (aa 1-71), followed by a mature region that possesses a catalytic domain between aa 109-440. There are three potential isoform variants. Two contain alternative start sites 25 and 59 aa upstream of the standard start site, respectively. A third shows a 12 aa substitution for aa 105-116. Over aa 72-537, human PPM2C shares 99% aa identity with mouse PPM2C.

Entrez Gene IDs

54704 (Human); 381511 (Mouse); 54705 (Rat)

Alternate Names

[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial; EC 3.1.3; EC 3.1.3.43; MGC119646; PDH; PDP 1; PDP1; PDPC 1; PDPC; PDPFLJ32517; PPM2C; PPM2CFLJ56179; Protein phosphatase 2C; protein phosphatase 2C, magnesium-dependent, catalytic subunit; pyruvate dehydrogenase (Lipoamide) phosphatase-phosphatase; Pyruvate dehydrogenase phosphatase catalytic subunit 1; Pyruvate Dehydrogenase Phosphatase; pyruvate dehyrogenase phosphatase catalytic subunit 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Murine Cytomegalovirus Spreads by Dendritic Cell Recirculation
Authors: HE Farrell, K Bruce, C Lawler, M Oliveira, R Cardin, N Davis-Poyn, PG Stevenson
MBio, 2017;8(5):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P