Human RAGE Quantikine ELISA Kit

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Control Products Available
Human RAGE ELISA Cell Culture Supernate Standard Curve
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Product Details
Procedure
Citations (38)
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Human RAGE Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
16.14 pg/mL
Assay Range
78.0 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human RAGE
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human RAGE Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human RAGE (extracellular domain) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human RAGE/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RAGE showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring RAGE.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 546 1527 3117 519 1449 2890
Standard Deviation 34 73 189 43 119 192
CV% 6.2 4.8 6.1 8.2 8.2 6.7

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 571 1549 3189 563 1568 3159
Standard Deviation 34 73 191 49 110 193
CV% 5.9 4.7 6 8.7 7 6.1

Recovery

The recovery of RAGE spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Tissue Culture Media (n=4) 98 86-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RAGE were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human RAGE ELISA Linearity

Data Examples

Human RAGE ELISA Cell Culture Supernate Standard Curve

Human RAGE ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: RAGE/AGER

RAGE (Receptor for Advanced Glycation End product) is a transmembrane glycoprotein that binds advanced glycation end products (AGEs), beta-amyloid peptides, HMGB1/Amphoterin, and several S100 family proteins. AGEs are adducts formed by the non-enzymatic glycation and oxidation of proteins and lipids. A soluble form can also be generated by MMP-mediated shedding. RAGE is expressed in the CNS during development as well as in adult endothelial cells, smooth muscle cells, pericytes, monocytes, and neurons. It is locally upregulated in vascular inflammation (e.g. diabetes, atherosclerosis, vascular injury, Alzheimer’s disease). At these sites, RAGE binding to S100A1, EN-RAGE/S100A12, or S100B induces inflammatory immune cell adhesion and infiltration as well as vascular smooth muscle proliferation, neointimal expansion, atherosclerotic plaque development, and transport of A-beta into the cerebrospinal fluid. In cancer, RAGE binding to HMGB1, S100A8, or S100A9 promotes tumor growth and metastasis in addition to inflammatory cell infiltration.

Long Name:
Receptor for Advanced Glycation End Products
Entrez Gene IDs:
177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)
Alternate Names:
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human RAGE Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Receptor for advanced glycation end-products and ARDS prediction: a multicentre observational study
    Authors: M Jabaudon, P Berthelin, T Pranal, L Roszyk, T Godet, JS Faure, R Chabanne, N Eisenmann, A Lautrette, C Belville, R Blondonnet, S Cayot, T Gillart, J Pascal, Y Skrzypczak, B Souweine, L Blanchon, V Sapin, B Pereira, JM Constantin
    Sci Rep, 2018;8(1):2603.
    Species: Human
    Sample Types: Plasma
  2. Elevations of novel cytokines in bacterial meningitis in infants
    Authors: L Srinivasan, L Kilpatrick, SS Shah, S Abbasi, MC Harris
    PLoS ONE, 2018;13(2):e0181449.
    Species: Human
    Sample Types: CSF
  3. TGF?1 reinforces arterial aging in the vascular smooth muscle cell through a long-range regulation of the cytoskeletal stiffness
    Authors: W Zhu, BC Kim, M Wang, J Huang, A Isak, NM Bexiga, R Monticone, T Ha, EG Lakatta, SS An
    Sci Rep, 2018;8(1):2668.
    Species: Human
    Sample Types: Plasma
  4. A prospective study of soluble receptor for advanced glycation end products and adipokines in association with pancreatic cancer in postmenopausal women
    Authors: DL White, RC Hoogeveen, L Chen, P Richardson, M Ravishanka, P Shah, L Tinker, T Rohan, EA Whitsel, HB El-Serag, L Jiao
    Cancer Med, 2018;0(0):.
    Species: Human
    Sample Types: Serum
  5. Club cell protein 16 and cytokeratin fragment 21-1 as early predictors of pulmonary complications in polytraumatized patients with severe chest trauma
    Authors: LL Negrin, G Halat, S Kettner, M Gregori, R Ristl, S Hajdu, T Heinz
    PLoS ONE, 2017;12(4):e0175303.
    Species: Human
    Sample Types: Serum
  6. Evolution and bad prognostic value of advanced glycation end products after acute heart failure: relation with body composition
    Authors: B Paradela-D, Á Fernández-, D Bou-Teen, S Eiras, R González-F, RM Agra, A Varela-Rom, AI Castro-Pai, MC Carreira, FF Casanueva, E Álvarez, JR González-J
    Cardiovasc Diabetol, 2017;16(1):115.
    Species: Human
    Sample Types: Plasma
  7. The Initial Months of Antiretroviral Therapy and Its Influence on AGEs, HMGB1, and sRAGE Levels in Asymptomatic HIV-Infected Individuals
    Authors: Karen Ingrid Tasca
    Mediators Inflamm, 2016;2016(0):2909576.
    Species: Human
    Sample Types: Plasma
  8. Decreased levels of sRAGE in follicular fluid from patients with PCOS
    Authors: BiJun Wang
    Reproduction, 2016;0(0):.
    Species: Human
    Sample Types: Aspirated Follicle Fluid
  9. Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
    PLoS ONE, 2016;11(8):e0160153.
    Species: Human
    Sample Types: Plasma
  10. Soluble Forms and Ligands of the Receptor for Advanced Glycation End-Products in Patients with Acute Respiratory Distress Syndrome: An Observational Prospective Study.
    Authors: Jabaudon M, Blondonnet R, Roszyk L, Pereira B, Guerin R, Perbet S, Cayot S, Bouvier D, Blanchon L, Sapin V, Constantin J
    PLoS ONE, 2015;10(8):e0135857.
    Species: Human
    Sample Types: Serum
  11. Serum Level of Soluble Receptor for Advanced Glycation End Products Is Associated with A Disintegrin And Metalloproteinase 10 in Type 1 Diabetes.
    Authors: Lee A, Lam J, Shiu S, Wong Y, Betteridge D, Tan K
    PLoS ONE, 2015;10(9):e0137330.
    Species: Human
    Sample Types: Serum
  12. Soluble RAGE and atherosclerosis in youth with type 1 diabetes: a 5-year follow-up study.
    Authors: Heier M, Margeirsdottir H, Gaarder M, Stensaeth K, Brunborg C, Torjesen P, Seljeflot I, Hanssen K, Dahl-Jorgensen K
    Cardiovasc Diabetol, 2015;14(1):126.
    Species: Human
    Sample Types: Serum
  13. The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
    Authors: Chou C, Lin F, Tsai H, Chang S
    BMC Pulm Med, 2014;14(0):182.
    Species: Human
    Sample Types: BALF
  14. Higher serum soluble receptor for advanced glycation end product levels and lower prevalence of metabolic syndrome among Japanese adult men: a cross-sectional study.
    Authors: Momma H, Niu K, Kobayashi Y, Huang C, Chujo M, Otomo A, Tadaura H, Miyata T, Nagatomi R
    Diabetol Metab Syndr, 2014;6(1):33.
    Species: Human
    Sample Types: Serum
  15. GeneOptimizer program-assisted cDNA reengineering enhances sRAGE autologous expression in Chinese hamster ovary cells.
    Authors: Wei, Wen, Kim, Ji Min, Medina, Danny, Lakatta, Edward G, Lin, Li
    Protein Expr Purif, 2014;95(0):143-8.
    Species: Chinese Hamster
    Sample Types: Cell Culture Supernates
  16. Local and systemic RAGE axis changes in pulmonary hypertension: CTEPH and iPAH.
    Authors: Moser B, Megerle A, Bekos C, Janik S, Szerafin T, Birner P, Schiefer A, Mildner M, Lang I, Skoro-Sajer N, Sadushi-Kolici R, Taghavi S, Klepetko W, Ankersmit H
    PLoS ONE, 2014;9(9):e106440.
    Species: Human
    Sample Types: Serum
  17. Placental growth factor, pregnancy-associated plasma protein-A, soluble receptor for advanced glycation end products, extracellular newly identified receptor for receptor for advanced glycation end products binding protein and high mobility group box 1 levels in patients with acute kidney injury: a cross sectional study.
    Authors: Zakiyanov O, Kriha V, Vachek J, Zima T, Tesar V, Kalousova M
    BMC Nephrol, 2013;14(0):245.
    Species: Human
    Sample Types: Serum
  18. The soluble form of the receptor of advanced glycation endproducts increases after bariatric surgery in morbid obesity.
    Authors: Brix J, Hollerl F, Kopp H, Schernthaner G, Schernthaner G
    Int J Obes (Lond), 2012;36(11):1412-7.
    Species: Human
    Sample Types: Serum
  19. Diastolic dysfunction of aging is independent of myocardial structure but associated with plasma advanced glycation end-product levels.
    Authors: Campbell D, Somaratne J, Jenkins A, Prior D, Yii M, Kenny J, Newcomb A, Schalkwijk C, Black M, Kelly D
    PLoS ONE, 2012;7(11):e49813.
    Species: Human
    Sample Types: Plasma
  20. Soluble RAGE as a severity marker in community acquired pneumonia associated sepsis.
    Authors: Narvaez-Rivera RM, Rendon A, Salinas-Carmona MC
    BMC Infect. Dis., 2012;12(0):15.
    Species: Human
    Sample Types: Serum
  21. Methodological and Preanalytical Evaluation of a RAGE Immunoassay.
    Authors: Wittwer C, Lehner J, Fersching D, Siegele B, Stoetzer OJ, Holdenrieder S
    Anticancer Res., 2012;32(5):2075-8.
    Species: Human
    Sample Types: Serum
  22. Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
    Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
    Species: Human
    Sample Types: Edema Fluid
  23. Soluble form of the receptor for advanced glycation end products is a marker of acute lung injury but not of severe sepsis in critically ill patients.
    Authors: Jabaudon M, Futier E, Roszyk L
    Crit. Care Med., 2011;39(3):480-8.
    Species: Human
    Sample Types: Plasma
  24. Total soluble and endogenous secretory receptor for advanced glycation end products as predictive biomarkers of coronary heart disease risk in patients with type 2 diabetes: an analysis from the CARDS trial.
    Authors: Colhoun HM, Betteridge DJ, Durrington P, Hitman G, Neil A, Livingstone S, Charlton-Menys V, Bao W, Demicco DA, Preston GM, Deshmukh H, Tan K, Fuller JH
    Diabetes, 2011;60(9):2379-85.
    Species: Human
    Sample Types: Serum
  25. Higher plasma soluble Receptor for Advanced Glycation End Products (sRAGE) levels are associated with incident cardiovascular disease and all-cause mortality in type 1 diabetes: a 12-year follow-up study.
    Authors: Nin JW, Jorsal A, Ferreira I
    Diabetes, 2010;59(8):2027-32.
    Species: Human
    Sample Types: Plasma
  26. Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes.
    Authors: Tam HL, Shiu SW, Wong Y, Chow WS, Betteridge DJ, Tan KC
    Atherosclerosis, 2010;209(1):173-7.
    Species: Human
    Sample Types: Serum
  27. Circulating high-molecular-weight RAGE ligands activate pathways implicated in the development of diabetic nephropathy.
    Authors: Penfold SA, Coughlan MT, Patel SK
    Kidney Int., 2010;78(3):287-95.
    Species: Human
    Sample Types: Serum
  28. Activation of critical, host-induced, metabolic and stress pathways marks neutrophil entry into cystic fibrosis lungs.
    Authors: Makam M, Diaz D, Laval J, Gernez Y, Conrad CK, Dunn CE, Davies ZA, Moss RB, Herzenberg LA, Herzenberg LA, Tirouvanziam R
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5779-83.
    Species: Human
    Sample Types: Plasma
  29. Serum-soluble receptor for advanced glycation end product levels in patients with amyotrophic lateral sclerosis.
    Acta Neurol. Scand., 2009;120(2):119-22.
    Species: Human
    Sample Types: Serum
  30. Characterization of RAGE, HMGB1, and S100beta in inflammation-induced preterm birth and fetal tissue injury.
    Authors: Buhimschi CS, Baumbusch MA, Dulay AT, Oliver EA, Lee S, Zhao G, Bhandari V, Ehrenkranz RA, Weiner CP, Madri JA, Buhimschi IA
    Am. J. Pathol., 2009;175(3):958-75.
    Species: Human
    Sample Types: Plasma
  31. Procoagulant alveolar microparticles in the lungs of patients with acute respiratory distress syndrome.
    Authors: Bastarache JA, Fremont RD, Kropski JA, Bossert FR, Ware LB
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(6):L1035-41.
    Species: Human
    Sample Types: Edema Fluid
  32. Serum endogenous secretory RAGE level is an independent risk factor for the progression of carotid atherosclerosis in type 1 diabetes.
    Authors: Katakami N, Matsuhisa M, Kaneto H, Matsuoka TA, Sakamoto K, Yasuda T, Umayahara Y, Kosugi K, Yamasaki Y
    Atherosclerosis, 2009;204(1):288-92.
    Species: Human
    Sample Types: Serum
  33. Increased serum HMGB1 level is associated with coronary artery disease in nondiabetic and type 2 diabetic patients.
    Authors: Yan XX, Lu L, Peng WH, Wang LJ, Zhang Q, Zhang RY, Chen QJ, Shen WF
    Atherosclerosis, 2009;205(2):544-8.
    Species: Human
    Sample Types: Serum
  34. Receptor for advanced glycation end products (RAGE) and its inflammatory ligand EN-RAGE in non-diabetic subjects with pre-mature coronary artery disease.
    Authors: Mahajan N, Malik N, Bahl A
    Atherosclerosis, 2009;207(2):597-602.
    Species: Human
    Sample Types: Plasma
  35. MMP1 and MMP7 as potential peripheral blood biomarkers in idiopathic pulmonary fibrosis.
    Authors: Rosas IO, Richards TJ, Konishi K, Zhang Y, Gibson K, Lokshin AE, Lindell KO, Cisneros J, Macdonald SD, Pardo A, Sciurba F, Dauber J, Selman M, Gochuico BR, Kaminski N
    PLoS Med., 2008;5(4):e93.
    Species: Human
    Sample Types: Serum
  36. Nitric oxide inhalation and glucocorticoids as combined treatment in human experimental endotoxemia.
    Authors: Hallstrom L, Berghall E, Frostell C, Sollevi A, Soop AL
    Crit. Care Med., 2008;36(11):3043-7.
    Species: Human
    Sample Types: Plasma
  37. Plasma receptor for advanced glycation end products and clinical outcomes in acute lung injury.
    Authors: Calfee CS, Ware LB, Eisner MD, Parsons PE, Thompson BT, Wickersham N, Matthay MA,
    Thorax, 2008;63(12):1083-9.
    Species: Human
    Sample Types: Plasma
  38. Low levels of sRAGE are associated with increased risk for mortality in renal transplant recipients.
    Authors: Gross S, van Ree RM, Oterdoom LH, de Vries AP, van Son WJ, de Jong PE, Navis GJ, Zuurman MW, Bierhaus A, Gans RO, Bakker SJ
    Transplantation, 2007;84(5):659-63.
    Species: Human
    Sample Types: Plasma

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Human RAGE Quantikine ELISA Kit
By Anonymous on 10/11/2017
Sample Tested: Plasma