• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    16.14 pg/mL
  • Assay Range
    78.0 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human RAGE
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC152, Quantikine Immunoassay Control Set 832 for Human RAGE - Please Inquire
Product Summary
The Quantikine Human RAGE Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human RAGE (extracellular domain) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human RAGE/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RAGE showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring RAGE.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean5711549318956315683159
Standard Deviation347319149110193
CV%5.94.768.776.1

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean5461527311751914492890
Standard Deviation347318943119192
CV%6.24.86.18.28.26.7

Recovery

The recovery of RAGE spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Tissue Culture Media (n=4) 98 86-108
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RAGE were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human RAGE Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: RAGE
RAGE (Receptor for Advanced Glycation End product) is a transmembrane glycoprotein that binds advanced glycation end products (AGEs), beta-amyloid peptides, HMGB1/Amphoterin, and several S100 family proteins. AGEs are adducts formed by the non-enzymatic glycation and oxidation of proteins and lipids. A soluble form can also be generated by MMP-mediated shedding. RAGE is expressed in the CNS during development as well as in adult endothelial cells, smooth muscle cells, pericytes, monocytes, and neurons. It is locally upregulated in vascular inflammation (e.g. diabetes, atherosclerosis, vascular injury, Alzheimer’s disease). At these sites, RAGE binding to S100A1, EN-RAGE/S100A12, or S100B induces inflammatory immune cell adhesion and infiltration as well as vascular smooth muscle proliferation, neointimal expansion, atherosclerotic plaque development, and transport of A-beta into the cerebrospinal fluid. In cancer, RAGE binding to HMGB1, S100A8, or S100A9 promotes tumor growth and metastasis in addition to inflammatory cell infiltration.
    • Long Name
      Receptor for Advanced Glycation End Products
    • Entrez Gene IDs
      177 (Human); 11596 (Mouse); 81722 (Rat);
    • Alternate Names
      AGER; EC 2.7.11.22; MOK protein kinase; MOKMAPK/MAK/MRK overlapping kinase; RAGE; RAGE-1; RAGE1renal cell carcinoma antigen (MOK protein kinase); Renal tumor antigen 1; renal tumor antigen;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    31 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Club cell protein 16 and cytokeratin fragment 21-1 as early predictors of pulmonary complications in polytraumatized patients with severe chest trauma
      Authors: LL Negrin, G Halat, S Kettner, M Gregori, R Ristl, S Hajdu, T Heinz
      PLoS ONE, 2017;12(4):e0175303.
      Species: Human
      Sample Type: Serum
    2. Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
      PLoS ONE, 2016;11(8):e0160153.
      Species: Human
      Sample Type: Plasma
    3. Serum Level of Soluble Receptor for Advanced Glycation End Products Is Associated with A Disintegrin And Metalloproteinase 10 in Type 1 Diabetes.
      Authors: Lee A, Lam J, Shiu S, Wong Y, Betteridge D, Tan K
      PLoS ONE, 2015;10(9):e0137330.
      Species: Human
      Sample Type: Serum
    4. Soluble RAGE and atherosclerosis in youth with type 1 diabetes: a 5-year follow-up study.
      Authors: Heier M, Margeirsdottir H, Gaarder M, Stensaeth K, Brunborg C, Torjesen P, Seljeflot I, Hanssen K, Dahl-Jorgensen K
      Cardiovasc Diabetol, 2015;14(1):126.
      Species: Human
      Sample Type: Serum
    5. Soluble Forms and Ligands of the Receptor for Advanced Glycation End-Products in Patients with Acute Respiratory Distress Syndrome: An Observational Prospective Study.
      Authors: Jabaudon M, Blondonnet R, Roszyk L, Pereira B, Guerin R, Perbet S, Cayot S, Bouvier D, Blanchon L, Sapin V, Constantin J
      PLoS ONE, 2015;10(8):e0135857.
      Species: Human
      Sample Type: Serum
    6. The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
      Authors: Chou C, Lin F, Tsai H, Chang S
      BMC Pulm Med, 2014;14(0):182.
      Species: Human
      Sample Type: BALF
    7. GeneOptimizer program-assisted cDNA reengineering enhances sRAGE autologous expression in Chinese hamster ovary cells.
      Authors: Wei, Wen, Kim, Ji Min, Medina, Danny, Lakatta, Edward G, Lin, Li
      Protein Expr Purif, 2014;95(0):143-8.
      Species: Chinese Hamster
      Sample Type: Cell Culture Supernates
    8. Higher serum soluble receptor for advanced glycation end product levels and lower prevalence of metabolic syndrome among Japanese adult men: a cross-sectional study.
      Authors: Momma H, Niu K, Kobayashi Y, Huang C, Chujo M, Otomo A, Tadaura H, Miyata T, Nagatomi R
      Diabetol Metab Syndr, 2014;6(1):33.
      Species: Human
      Sample Type: Serum
    9. Local and systemic RAGE axis changes in pulmonary hypertension: CTEPH and iPAH.
      Authors: Moser B, Megerle A, Bekos C, Janik S, Szerafin T, Birner P, Schiefer A, Mildner M, Lang I, Skoro-Sajer N, Sadushi-Kolici R, Taghavi S, Klepetko W, Ankersmit H
      PLoS ONE, 2014;9(9):e106440.
      Species: Human
      Sample Type: Serum
    10. Placental growth factor, pregnancy-associated plasma protein-A, soluble receptor for advanced glycation end products, extracellular newly identified receptor for receptor for advanced glycation end products binding protein and high mobility group box 1 levels in patients with acute kidney injury: a cross sectional study.
      Authors: Zakiyanov O, Kriha V, Vachek J, Zima T, Tesar V, Kalousova M
      BMC Nephrol, 2013;14(0):245.
      Species: Human
      Sample Type: Serum
    11. Diastolic dysfunction of aging is independent of myocardial structure but associated with plasma advanced glycation end-product levels.
      Authors: Campbell D, Somaratne J, Jenkins A, Prior D, Yii M, Kenny J, Newcomb A, Schalkwijk C, Black M, Kelly D
      PLoS ONE, 2012;7(11):e49813.
      Species: Human
      Sample Type: Plasma
    12. Soluble RAGE as a severity marker in community acquired pneumonia associated sepsis.
      Authors: Narvaez-Rivera RM, Rendon A, Salinas-Carmona MC
      BMC Infect. Dis., 2012;12(0):15.
      Species: Human
      Sample Type: Serum
    13. Methodological and Preanalytical Evaluation of a RAGE Immunoassay.
      Authors: Wittwer C, Lehner J, Fersching D, Siegele B, Stoetzer OJ, Holdenrieder S
      Anticancer Res., 2012;32(5):2075-8.
      Species: Human
      Sample Type: Serum
    14. The soluble form of the receptor of advanced glycation endproducts increases after bariatric surgery in morbid obesity.
      Authors: Brix J, Hollerl F, Kopp H, Schernthaner G, Schernthaner G
      Int J Obes (Lond), 2012;36(11):1412-7.
      Species: Human
      Sample Type: Serum
    15. Soluble form of the receptor for advanced glycation end products is a marker of acute lung injury but not of severe sepsis in critically ill patients.
      Authors: Jabaudon M, Futier E, Roszyk L
      Crit. Care Med., 2011;39(3):480-8.
      Species: Human
      Sample Type: Plasma
    16. Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
      Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
      Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
      Species: Human
      Sample Type: Edema Fluid
    17. Total soluble and endogenous secretory receptor for advanced glycation end products as predictive biomarkers of coronary heart disease risk in patients with type 2 diabetes: an analysis from the CARDS trial.
      Authors: Colhoun HM, Betteridge DJ, Durrington P, Hitman G, Neil A, Livingstone S, Charlton-Menys V, Bao W, Demicco DA, Preston GM, Deshmukh H, Tan K, Fuller JH
      Diabetes, 2011;60(9):2379-85.
      Species: Human
      Sample Type: Serum
    18. Circulating high-molecular-weight RAGE ligands activate pathways implicated in the development of diabetic nephropathy.
      Authors: Penfold SA, Coughlan MT, Patel SK
      Kidney Int., 2010;78(3):287-95.
      Species: Human
      Sample Type: Serum
    19. Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes.
      Authors: Tam HL, Shiu SW, Wong Y, Chow WS, Betteridge DJ, Tan KC
      Atherosclerosis, 2010;209(1):173-7.
      Species: Human
      Sample Type: Serum
    20. Higher plasma soluble Receptor for Advanced Glycation End Products (sRAGE) levels are associated with incident cardiovascular disease and all-cause mortality in type 1 diabetes: a 12-year follow-up study.
      Authors: Nin JW, Jorsal A, Ferreira I
      Diabetes, 2010;59(8):2027-32.
      Species: Human
      Sample Type: Plasma
    21. Receptor for advanced glycation end products (RAGE) and its inflammatory ligand EN-RAGE in non-diabetic subjects with pre-mature coronary artery disease.
      Authors: Mahajan N, Malik N, Bahl A
      Atherosclerosis, 2009;207(2):597-602.
      Species: Human
      Sample Type: Plasma
    22. Activation of critical, host-induced, metabolic and stress pathways marks neutrophil entry into cystic fibrosis lungs.
      Authors: Makam M, Diaz D, Laval J, Gernez Y, Conrad CK, Dunn CE, Davies ZA, Moss RB, Herzenberg LA, Herzenberg LA, Tirouvanziam R
      Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5779-83.
      Species: Human
      Sample Type: Plasma
    23. Characterization of RAGE, HMGB1, and S100beta in inflammation-induced preterm birth and fetal tissue injury.
      Authors: Buhimschi CS, Baumbusch MA, Dulay AT, Oliver EA, Lee S, Zhao G, Bhandari V, Ehrenkranz RA, Weiner CP, Madri JA, Buhimschi IA
      Am. J. Pathol., 2009;175(3):958-75.
      Species: Human
      Sample Type: Plasma
    24. Increased serum HMGB1 level is associated with coronary artery disease in nondiabetic and type 2 diabetic patients.
      Authors: Yan XX, Lu L, Peng WH, Wang LJ, Zhang Q, Zhang RY, Chen QJ, Shen WF
      Atherosclerosis, 2009;205(2):544-8.
      Species: Human
      Sample Type: Serum
    25. Serum-soluble receptor for advanced glycation end product levels in patients with amyotrophic lateral sclerosis.
      Acta Neurol. Scand., 2009;120(2):119-22.
      Species: Human
      Sample Type: Serum
    26. Procoagulant alveolar microparticles in the lungs of patients with acute respiratory distress syndrome.
      Authors: Bastarache JA, Fremont RD, Kropski JA, Bossert FR, Ware LB
      Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(6):L1035-41.
      Species: Human
      Sample Type: Edema Fluid
    27. Serum endogenous secretory RAGE level is an independent risk factor for the progression of carotid atherosclerosis in type 1 diabetes.
      Authors: Katakami N, Matsuhisa M, Kaneto H, Matsuoka TA, Sakamoto K, Yasuda T, Umayahara Y, Kosugi K, Yamasaki Y
      Atherosclerosis, 2009;204(1):288-92.
      Species: Human
      Sample Type: Serum
    28. MMP1 and MMP7 as potential peripheral blood biomarkers in idiopathic pulmonary fibrosis.
      Authors: Rosas IO, Richards TJ, Konishi K, Zhang Y, Gibson K, Lokshin AE, Lindell KO, Cisneros J, Macdonald SD, Pardo A, Sciurba F, Dauber J, Selman M, Gochuico BR, Kaminski N
      PLoS Med., 2008;5(4):e93.
      Species: Human
      Sample Type: Serum
    29. Nitric oxide inhalation and glucocorticoids as combined treatment in human experimental endotoxemia.
      Authors: Hallstrom L, Berghall E, Frostell C, Sollevi A, Soop AL
      Crit. Care Med., 2008;36(11):3043-7.
      Species: Human
      Sample Type: Plasma
    30. Plasma receptor for advanced glycation end products and clinical outcomes in acute lung injury.
      Authors: Calfee CS, Ware LB, Eisner MD, Parsons PE, Thompson BT, Wickersham N, Matthay MA,
      Thorax, 2008;63(12):1083-9.
      Species: Human
      Sample Type: Plasma
    31. Low levels of sRAGE are associated with increased risk for mortality in renal transplant recipients.
      Authors: Gross S, van Ree RM, Oterdoom LH, de Vries AP, van Son WJ, de Jong PE, Navis GJ, Zuurman MW, Bierhaus A, Gans RO, Bakker SJ
      Transplantation, 2007;84(5):659-63.
      Species: Human
      Sample Type: Plasma
    Expand to show all 31 Citations
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 832 for Human RAGE

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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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