Human RANK/TNFRSF11A Antibody

Clone 80704 was used by HLDA to establish CD designation.
(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human RANK/TNFRSF11A in ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymphotoxin beta R, rhNGF R, rhOPG, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse (rm) OX40, and rmRANK is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 80704
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human RANK/TNFRSF11A. extracellular domain
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human RANK/TNFRSF11A Fc Chimera (Catalog # 683-RK)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Human RANK/TNFRSF11A Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human RANK/TNFRSF11A Antibody (Catalog # MAB683)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human RANK/TNFRSF11A Biotinylated Antibody (Catalog # BAF683)
  • ELISA Standard
     
    Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF (Catalog # 683-RK)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of RANK/TNFRSF11A in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB683, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

  • References:
    1. Anderson, D.M. et al. (1997) Nature 390:175.
    2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  • Long Name:
    Receptor Activator of NF-kappa-B
  • Entrez Gene IDs:
    8792 (Human); 21934 (Mouse)
  • Alternate Names:
    CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Langerhans cell precursors acquire RANK/CD265 in prenatal human skin.
    Authors: Schoppl A, Botta A, Prior M, Akgun J, Schuster C, Elbe-Burger A
    Acta Histochem, 2015;117(4):425-30.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Receptor activator for NF-kappaB ligand in acute myeloid leukemia: expression, function, and modulation of NK cell immunosurveillance.
    Authors: Schmiedel B, Nuebling T, Steinbacher J, Malinovska A, Wende C, Azuma M, Schneider P, Grosse-Hovest L, Salih H
    J Immunol, 2013;190(2):821-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. RANKL expression, function, and therapeutic targeting in multiple myeloma and chronic lymphocytic leukemia.
    Authors: Schmiedel B, Scheible C, Nuebling T, Kopp H, Wirths S, Azuma M, Schneider P, Jung G, Grosse-Hovest L, Salih H
    Cancer Res, 2013;73(2):683-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Regulatory T cells in the bone marrow microenvironment in patients with prostate cancer.
    Authors: Zhao E, Wang L, Dai J, Kryczek I, Wei S, Vatan L, Altuwaijri S, Sparwasser T, Wang G, Keller E, Zou W
    Oncoimmunology, 2012;1(2):152-161.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation.
    Authors: Barbaroux JB, Beleut M, Brisken C, Mueller CG, Groves RW
    J. Immunol., 2008;181(2):1103-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. High expression levels of receptor activator of nuclear factor-kappa B ligand associated with human chronic periodontitis are mainly secreted by CD4+ T lymphocytes.
    Authors: Vernal R, Dutzan N, Hernandez M, Chandia S, Puente J, Leon R, Garcia L, Del Valle I, Silva A, Gamonal J
    J. Periodontol., 2006;77(10):1772-80.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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