Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

Catalog # Availability Size / Price Qty
AF3960
AF3960-SP
Detection of Human and Rat  beta -1,3-N-acetylglucosaminyl­transferase 2/B3GNT2 by Western Blot.
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Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2
Lys29-Cys397
Accession # Q9NY97
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Rat  beta -1,3-N-acetylglucosaminyl­transferase 2/B3GNT2 by Western Blot. View Larger

Detection of Human and Rat  beta -1,3-N-acetylglucosaminyl­transferase 2/B3GNT2 by Western Blot. Western blot shows lysates of human brain tissue and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human beta ‑1,3‑N‑acetylglucosaminyltransferase 2/B3GNT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for beta ‑1,3‑N‑acetylglucosaminyltransferase 2/B3GNT2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2

B3-GNT (also B3GNT-2) is an 80 kDa (45 kDa without glycosylation) type II transmembrane  Golgi enzyme that belongs to the glycosyltransferase 31 family of enzymes. It catalyzes the transfer of N-acetyl glucosamine from UDP-GlcNAc onto Gal beta 3(GlcNAc beta 6)GalNAc-mucin. It functions in the elongation and branching of O-linked oligosaccharide chains of mucin glycoproteins. Over aa 29-397, human B3GNT2 shares 88% aa sequence identity with rat B3GNT2.

Long Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 1
Entrez Gene IDs
10678 (Human); 53625 (Mouse)
Alternate Names
B3Galt6; B3GALT7; b3Gal-T7; B3GNT; B3GNT1; B3GN-T1; B3GNT2; B3GN-T2; B3GNT-2; Beta-1,3-galactosyltransferase 7; Beta-1,3-GalTase 7; Beta-1,3-Gn-T1; Beta-1,3-Gn-T2; Beta-1,3-N-acetylglucosaminyltransferase 1; Beta-1,3-N-acetylglucosaminyltransferase 2; beta-1,3-N-acetylglucosaminyltransferase bGnT-1; beta-1,3-N-acetylglucosaminyltransferase bGnT-2; Beta3GalT7; Beta3Gal-T7; BETA3GNT; Beta3gnt1; Beta3Gn-T1; Beta3Gn-T2; Beta-3-Gx-T7; BGnT-1; BGNT2; BGnT-2; EC 2.4.1.-; UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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