Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody Summary
Accession # Q9NY97
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Rat beta -1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 by Western Blot. Western blot shows lysates of human brain tissue and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2
B3-GNT (also B3GNT-2) is an 80 kDa (45 kDa without glycosylation) type II transmembrane Golgi enzyme that belongs to the glycosyltransferase 31 family of enzymes. It catalyzes the transfer of N-acetyl glucosamine from UDP-GlcNAc onto Gal beta 3(GlcNAc beta 6)GalNAc-mucin. It functions in the elongation and branching of O-linked oligosaccharide chains of mucin glycoproteins. Over aa 29-397, human B3GNT2 shares 88% aa sequence identity with rat B3GNT2.
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