Human/Rat CaMKK alpha Antibody

  
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects endogenous human and rat CaMKK alpha in Western blots. In Western blots, this antibody does not cross-react with recombinant human CaMKK beta.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CaMKK alpha
    Met1-Ser132
    Accession # Q8N5S9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Rat CaMKK alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, K562 human chronic myelogenous leukemia cell line, and human brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Rat CaMKK alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4536) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaMKK alpha at approximately 68 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Detection of Human CaMKK alpha by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CaMKK alpha at approximately 68 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat CaMKK alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4536) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CaMKK alpha

The calcium/calmodulin (CaM) kinase signaling cascade includes the upstream kinases CaM kinase kinase alpha (CaMKK alpha ; also known as CaMKK1) and CaMKK beta (CaMKK2), and their substrates, the output kinases CaMKI and CaMKIV. Largely by activation of the transcription factor CREB, the CaM kinase cascade is implicated in neuronal development and long-term memory formation. In addition, CaMKK alpha is known to modulate skeletal muscle glucose uptake, and is upregulated during retinoic acid-induced neutrophil maturation.

  • Long Name:
    Calcium/Calmodulin-dependent Protein Kinase Kinase alpha
  • Entrez Gene IDs:
    84254 (Human); 55984 (Mouse); 60341 (Rat)
  • Alternate Names:
    calcium/calmodulin-dependent protein kinase kinase 1; calcium/calmodulin-dependent protein kinase kinase 1, alpha; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM-kinase IV kinase; CaM-kinase kinase 1; CaM-kinase kinase alpha; CaM-KK 1; CAMKK alpha protein; CaMKK alpha; CaM-KK alpha; CaMKK1; CAMKKACaMKK 1; DKFZp761M0423; EC 2.7.11; EC 2.7.11.17; MGC34095
Related Research Areas
Isotype Controls
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Secondary Antibodies
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Sheep IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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