|Detection of Human and Rat M‑Ras/R‑Ras3 by Western Blot. Western blot shows lysates of L6 rat myoblast cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat M‑Ras/R‑Ras3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R‑Ras, TC21/R-Ras2, and M‑Ras/R‑Ras3 (2 ng/lane) were included. A specific band for M‑Ras/R‑Ras3 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Muscle Ras (M-Ras), also known as R-Ras3, is a small GTP-binding protein of the Ras superfamily of GTPases. M-Ras comprises an R-Ras subfamily with fellow members R-Ras and TC21 (R-Ras2). While highly homologous elsewhere, M-Ras differs at its carboxyl terminus from both R-Ras and TC21, suggesting this GTPase may interact with novel signaling effectors.
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