Detects both the pro and mature forms of human Relaxin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Relaxin-1 or rhRelaxin-3 is observed.
Monoclonal Rat IgG1 Clone # 349102
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Relaxin-2 Val23-Cys185 Accession # P04090
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize Relaxin‑2-induced cAMP production in the THP‑1 human acute monocytic leukemia cell line. Parsell, D. A. et al. (1996) J. Biol. Chem. 271:27936. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 7.5 ng/mL Recombinant Human Relaxin‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of Human Relaxin-2 by Western Blot.
Western blot shows lysates of human placenta. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Relaxin‑2 Monoclonal Antibody (Catalog # MAB2804) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Relaxin-2 at approximately 8 kDa and 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
cAMP production Induced by Relaxin‑2 and Neutralization by Human Relaxin‑2 Antibody.
Recombinant Human Relaxin‑2 (Catalog # 3596-RN) induces cAMP production in the THP‑1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line), as measured by cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recombinant Human Relaxin‑2 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human Relaxin‑2 Monoclonal Antibody (Catalog # MAB2804). The ND50 is typically 1.5‑6.0 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human Relaxin-2/H2 is a 6 kDa member of the insulin/relaxin gene superfamily. Relaxin-2 is synthesized as a 185 aa preprohormone that is proteolytically processed into a nonglycosylated, disulfide-linked, 53 aa mature heterodimer that contains a 29 aa B chain (aa 25‑53) and a 24 aa A chain (aa 161‑185). An alternate splice form of human Relaxin-2 is known that contains the B chain and an uncleaved 47 aa extension. Mature human Relaxin-2 heterodimer is 48%, 44% and 43% aa identical to rat, canine and porcine Relaxin-2, respectively.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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