Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Rictor Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rictor
Met1-Leu250
Accession # Q6R327

Specificity

Detects endogenous human Rictor in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Rictor Antibody

Detection of Human Rictor antibody by Western Blot.

Detection of Human Rictor by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Rictor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4598) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Rictor at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Rictor Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rictor

Rictor (rapamycin-insensitive companion of mTOR) is a member of the mTORC2 complex, which regulates cell growth by integrating stress, energy, and nutrient signals with growth factor derived signals. Rictor contains no obvious catalytic motifs, but knockdown studies of mTOR or Rictor results in a loss actin polymerization and cell spreading. In vitro, the mTOR-Rictor complex phosphorylates Akt/PKB on Ser473, an essential step for full activation of Akt/PKB. Additionally, recent studies have shown that mTOR-Rictor regulates PKC alpha phosphorylation and PDGFR beta expression and signaling.

Long Name

Rapamycin-insensitive Companion of mTOR

Alternate Names

mAVO3, Pianissimo

Entrez Gene IDs

253260 (Human)

Gene Symbol

RICTOR

UniProt

Q6R327

Additional Rictor Products

Product Documents for Human Rictor Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Rictor Antibody

For research use only

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