Human Rictor Antibody

Catalog # Availability Size / Price Qty
AF4598
AF4598-SP
Detection of Human Rictor by Western Blot.
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Human Rictor Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human Rictor in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Rictor
Met1-Leu250
Accession # Q6R327
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Rictor by Western Blot. View Larger

Detection of Human Rictor by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Rictor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4598) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Rictor at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rictor

Rictor (rapamycin-insensitive companion of mTOR) is a member of the mTORC2 complex, which regulates cell growth by integrating stress, energy, and nutrient signals with growth factor derived signals. Rictor contains no obvious catalytic motifs, but knockdown studies of mTOR or Rictor results in a loss actin polymerization and cell spreading. In vitro, the mTOR-Rictor complex phosphorylates Akt/PKB on Ser473, an essential step for full activation of Akt/PKB. Additionally, recent studies have shown that mTOR-Rictor regulates PKC alpha phosphorylation and PDGFR beta expression and signaling.

Long Name
Rapamycin-insensitive Companion of mTOR
Entrez Gene IDs
253260 (Human)
Alternate Names
AVO3 homolog; AVO3; hAVO3; KIAA1999DKFZp686B11164; mAVO3; MGC39830; PIA; Pianissimo; rapamycin-insensitive companion of mTOR; Rictor; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3

Product Datasheets

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