Human Semaphorin 3A Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (1)
Supplemental Products

Human Semaphorin 3A Antibody Summary

Species Reactivity
Detects human Semaphorin 3A in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Semaphorin 3B, rhSemaphorin 3C, rhSemaphorin 3D, rhSemaphorin 3E and rhSemaphorin 3F is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3A
Lys26-Val771 (Arg552Ala, Arg555Ala)
Accession # Q14563
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3)
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human T cells treated with PHA

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 3A

The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2).

Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2-like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1‑3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).

  1. Nakamura, F. et al. (2000) J Neurobiol. 44:219.
  2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
  3. Kolodkin, A.L. et al. (1993) Cell 75:1389.
  4. Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.
  5. Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.
  6. Luo Y. et al. (1993) Cell 75:217.
Entrez Gene IDs
10371 (Human); 20346 (Mouse); 29751 (Rat); 100620398 (Porcine); 102126862 (Cynomolgus Monkey)
Alternate Names
(semaphorin) 3A; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema III; SEMA1; Sema3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; semaphorin D; Semaphorin III; semaphorin L; semaphorin-3A; SemD

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Citation for Human Semaphorin 3A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Possible implication of satellite cells in regenerative motoneuritogenesis: HGF upregulates neural chemorepellent Sema3A during myogenic differentiation.
    Authors: Tatsumi R, Sankoda Y, Anderson JE, Sato Y, Mizunoya W, Shimizu N, Suzuki T, Yamada M, Rhoads RP, Ikeuchi Y, Allen RE
    Am. J. Physiol., Cell Physiol., 2009;297(2):C238-52.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot


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