Serpin B8 (serine proteinase inhibitor‑clade B #8; also PI8) is a 42‑45 kDa, cytoplasmic and secreted member of the ovalbumin (clade B)‑subfamily, Serpin superfamily of protease inhibitors. It is produced by multiple cell types and shows inhibitory activity towards furin and other proteinases such as chymotrypsin. Human Serpin B8 is 374 amino acids (aa) in length. Although it has no signal sequence, it is released by platelets. The active inhibitor site lies between Arg336 and Arg342. One potential isoform shows a 2 aa substitution for the C‑terminal 134 amino acids. Full length human Serpin B8 shares 78% and 83% aa identity with mouse and canine Serpin B8, respectively.
Human Serpin B8/Proteinase Inhibitor 8 Antibody
R&D Systems | Catalog # AF4158
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin B8/Proteinase Inhibitor 8
Asp2-Pro374
Accession # P50452
Asp2-Pro374
Accession # P50452
Specificity
Detects human Serpin B8/Proteinase Inhibitor 8 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Serpin B8 is observed and less than 2% cross-reactivity with recombinant human (rh) Serpin B6, rhSerpin B9, and rhSerpin A5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Serpin B8/Proteinase Inhibitor 8 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin B8/Proteinase Inhibitor 8, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin B8/Proteinase Inhibitor 8, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Serpin B8/Proteinase Inhibitor 8
Sample: Recombinant Human Serpin B8/Proteinase Inhibitor 8
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Serpin B8/Proteinase Inhibitor 8
Alternate Names
CAP2, NK10, PI8
Gene Symbol
SERPINB8
UniProt
Additional Serpin B8/Proteinase Inhibitor 8 Products
Product Documents for Human Serpin B8/Proteinase Inhibitor 8 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Serpin B8/Proteinase Inhibitor 8 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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