Human SHANK2 Antibody

Catalog # Availability Size / Price Qty
AF7035
AF7035-SP
Human SHANK2 Antibody in Immunohistochemistry
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human SHANK2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SHANK2 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SHANK2
Lys849-Thr1029
Accession # Q9UPX8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry SHANK2 in Human Brain. View Larger

SHANK2 in Human Brain. SHANK2 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human SHANK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7035) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and processes in Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHANK2

SHANK2 (SH3 and multiple ANKyrin repeat domain protein 2; also CortBP1 and ProSAP1) is a 180‑200 kDa member of the Shank family of proteins. It is expressed in neurons and various epithelial cells, and serves as a multidomain scaffold for cell membrane proteins. For example, in the PSD of neurons, it provides support for the interaction between glutamate receptors and the actin cytoskeleton. In epithelium, SHANK2 regulates the trafficking and activity of Na/H Exchanger 3 (NHE3). Human SHANK2 (sometimes known as ProSAP1) is 1470 amino acids (aa) in length. It contains an N‑terminal SH3 domain (aa 147‑206), one PDZ domain (aa 247‑341), a Pro‑rich region (aa 509‑535), one potential O‑linked glycosylation site (aa 948‑954) and a C‑terminal SAM domain (aa 1407‑1470). There are seven potential Ser/Thr phosphorylation sites. Multiple splice forms exist. There is a 220‑240 kDa ankyrin repeat‑containing isoform (SHANK2‑E) that possesses a 391 aa substitution for aa 1‑12, and a 165 kDa isoform (potentially known as CortBP1) that shows a 28 aa substitution for aa 1‑238 coupled with a deletion of aa 383‑390. Another potential isoform shows the previously noted 28 aa substitution for aa 1‑238 coupled to a 19 aa substitution for aa 472‑1470. Over aa 849‑1029, human SHANK2 shares 80% aa identity with mouse SHANK2.

Long Name
SH3 and Multiple Ankyrin Repeat Domains Protein 2
Entrez Gene IDs
22941 (Human); 210274 (Mouse)
Alternate Names
AUTS17; cortactin binding protein 1; cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CORTBP1; CORTBP1CTTNBP1; CTTNBP1; GKAP/SAPAP interacting protein; KIAA1022; proline-rich synapse associated protein 1; Proline-rich synapse-associated protein 1; ProSAP1; SH3 and multiple ankyrin repeat domains 2; SH3 and multiple ankyrin repeat domains protein 2; SHANK; SHANK2; SPANK-3

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human SHANK2 Antibody

There are currently no reviews for this product. Be the first to review Human SHANK2 Antibody and earn rewards!

Have you used Human SHANK2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review