Human SHARPIN Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human SHARPIN in direct ELISAs and Western blots. 
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SHARPIN
    Ala27-Ala182
    Accession # Q9H0F6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SHARPIN by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SHARPIN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8100) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SHARPIN at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
SHARPIN in Human Ovary. SHARPIN was detected in immersion fixed paraffin-embedded sections of human ovary using Sheep Anti-Human SHARPIN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8100) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SHARPIN
Sharpin (Shank-associated RH domain-interacting protein), also called SIPL1 (Shank interacting protein-like 1) is a 40-45kDa protein. It is an essential component of the cytosolic LUBAC ubiquitin conjugation complex. It conjugates linear ubiquitin chains to IKK gamma and activates NFkB, thus regulating immune and inflammatory responses. Sharpin is widely expressed, with upregulated expression in multiple tumor types. Human Sharpin is a 387 amino acid (aa) protein containing self association, SHANK1 interaction, and zinc finger domains. A 326 aa isoform diverges after aa 308 and lacks the zinc finger domain. Within aa 27-182, human Sharpin shares 67 and 66% aa sequence identity with mouse and rat Sharpin, respectively.
  • Long Name:
    SHANK-associated RH Domain Interactor
  • Entrez Gene IDs:
    81858 (Human); 106025 (Mouse); 81859 (Rat)
  • Alternate Names:
    DKFZp434N1923; hSIPL1; SHANK-associated RH domain interactor; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; SHARPIN; SIPL1; SIPL1DKFZP434N1923
Related Research Areas
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Isotype Controls
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Secondary Antibodies
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Sheep IgG (H+L) PE-conjugated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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