Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Sirtuin 2/SIRT2 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Sirtuin 2/SIRT2
Ala2-Gln389 (predicted)
Accession # Q8IXJ6

Specificity

Detects human Sirtuin 2/SIRT2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human SIRT1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Sirtuin 2/SIRT2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sirtuin 2/SIRT2

Sirtuin 2, encoded by the SIRT2 gene, is also known as SIR2 (silent information regulator 2)-like protein 2. It is a nicotinamide adenine dinucleotide (NAD)-dependent histone/protein deacetylase (1‑2). The SIR2 family of enzymes is classified as class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging (3‑4). Unlike class I and class II HDACs, the enzymatic activity of class III HDACs is NAD dependent and insensitive to HDAC inhibitor trichostatin A (5).

References

  1. Frye, R.A. (1999) Biochem. Biophys. Res. Commun. 260:273.
  2. North, B.J. et al. (2003) Mol. Cell. 11:437.
  3. North, B.J. and Verdin, E. (2004) Genome Biol. 5:224.
  4. Marmorstein, R. (2004) Biochem. Soc. Trans. 32:904.
  5. Gray, S.G. and Ekstrom, T.J. (2001) Exp. Cell Res. 262:75.  

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2L2, SIRT2

Entrez Gene IDs

22933 (Human); 64383 (Mouse); 361532 (Rat)

Gene Symbol

SIRT2

UniProt

Q8IXJ6

Additional Sirtuin 2/SIRT2 Products

Product Documents for Human Sirtuin 2/SIRT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Sirtuin 2/SIRT2 Antibody

For research use only

Related Research Areas

Autophagy
Epigenetics
Redox Enzymes

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