Detection of Human SKAP55 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human SKAP55 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4264) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human SKAP55 (1 ng) was included. A specific band for SKAP55 was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Src kinase-associated phosphoprotein of 55 kDa (SKAP55) is an adaptor protein expressed exclusively in mononuclear cells of hematopoietic lineage, with preferential expression in T lymphocytes and monocytes. Also known as SCAP1, SKAP55 regulates integrin-mediated adhesion and promotes synapse formation between T cells and antigen-presenting cells. SKAP55 is expressed as a constitutively tyrosine-phosphorylated protein that interacts with the SH2 domain of Fyn in resting cells.
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