Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SKAP55 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SKAP55
Met1-Arg358
Accession # NP_001068567

Specificity

Detects human SKAP55 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SKAP55 Antibody

Detection of Human SKAP55 antibody by Western Blot.

Detection of Human SKAP55 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human SKAP55 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4264) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human SKAP55 (1 ng) was included. A specific band for SKAP55 was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SKAP55 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SKAP55

Src kinase-associated phosphoprotein of 55 kDa (SKAP55) is an adaptor protein expressed exclusively in mononuclear cells of hematopoietic lineage, with preferential expression in T lymphocytes and monocytes. Also known as SCAP1, SKAP55 regulates integrin-mediated adhesion and promotes synapse formation between T cells and antigen-presenting cells. SKAP55 is expressed as a constitutively tyrosine-phosphorylated protein that interacts with the SH2 domain of Fyn in resting cells.

Long Name

Src Kinase-associated Phosphoprotein of 55 kDa

Alternate Names

SCAP1, SKAP1

Entrez Gene IDs

8631 (Human); 78473 (Mouse)

Gene Symbol

SKAP1

UniProt

NP_001068567

Additional SKAP55 Products

Product Documents for Human SKAP55 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SKAP55 Antibody

For research use only

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