Human SKAP55 Antibody

Catalog # Availability Size / Price Qty
AF4264
AF4264-SP
Detection of Human SKAP55 by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human SKAP55 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SKAP55 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SKAP55
Met1-Arg358
Accession # NP_001068567
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human SKAP55 by Western Blot. View Larger

Detection of Human SKAP55 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human SKAP55 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4264) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human SKAP55 (1 ng) was included. A specific band for SKAP55 was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SKAP55

Src kinase-associated phosphoprotein of 55 kDa (SKAP55) is an adaptor protein expressed exclusively in mononuclear cells of hematopoietic lineage, with preferential expression in T lymphocytes and monocytes. Also known as SCAP1, SKAP55 regulates integrin-mediated adhesion and promotes synapse formation between T cells and antigen-presenting cells. SKAP55 is expressed as a constitutively tyrosine-phosphorylated protein that interacts with the SH2 domain of Fyn in resting cells.

Long Name
Src Kinase-associated Phosphoprotein of 55 kDa
Entrez Gene IDs
8631 (Human); 78473 (Mouse)
Alternate Names
SCAP1; SCAP1pp55; SKAP1; SKAP55; SKAP55SKAP-55; src family associated phosphoprotein 1; Src family-associated phosphoprotein 1; src kinase associated phosphoprotein 1; src kinase-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55 kDa

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human SKAP55 Antibody

There are currently no reviews for this product. Be the first to review Human SKAP55 Antibody and earn rewards!

Have you used Human SKAP55 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review