Human SMPD3 Antibody

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Detection of Human SMPD3 by Western Blot.
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Product Details
Citations (1)
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Human SMPD3 Antibody Summary

Species Reactivity
Detects human SMPD3 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human SMPD3
Accession # Q9NY59
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SMPD3 antibody by Western Blot. View Larger

Detection of Human SMPD3 by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SMPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7184) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for SMPD3 at approximately 70 to 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMPD3

SMPD3 (Sphingomyelin phosphodiesterase 3; also Neutral sphingomyelinase 2/nSMase2) is a 69-74 kDa member of the neutral sphingomyelinase family of enzymes. It is a monomeric Golgi/plasma membrane enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide and phosphorylcholine. This generates second messenger components that participate in signal transduction. Human SMPD3 is a two transmembrane, 655 amino acids molecule. It contains an N-terminal luminal segment (aa 1-10), a cytoplasmic region (aa 32-64), and one catalytic domain (aa 340-646). Phosphorylation increases its MW to 78 kDa in SDS-PAGE. There is one potential isoform that possesses an Asn substitution for aa 569-587. Over aa 2-655, human SMPD3 shares 91% aa sequence identity with mouse SMPD3.

Long Name
Sphingomyelin Phosphodiesterase 3
Entrez Gene IDs
55512 (Human); 58994 (Mouse); 94338 (Rat)
Alternate Names
EC; FLJ22593; MGC138443; Neutral sphingomyelinase 2; Neutral sphingomyelinase II; NSMASE2; nSMase-2; SMPD3; sphingomyelin phosphodiesterase 3; sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinaseII)

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Citation for Human SMPD3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Intracellular localization of diacylglycerols and sphingolipids influences insulin sensitivity and mitochondrial function in human skeletal muscle
    Authors: L Perreault, SA Newsom, A Strauss, A Kerege, DE Kahn, KA Harrison, JK Snell-Berg, T Nemkov, A D'Alessand, MR Jackman, PS MacLean, BC Bergman
    JCI Insight, 2018-02-08;3(3):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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