Detection of Human SMPD3 by Western Blot.
Western blot shows lysates of RPMI 8226 human multiple myeloma cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SMPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7184) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for SMPD3 at approximately 70 to 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SMPD3 (Sphingomyelin phosphodiesterase 3; also Neutral sphingomyelinase 2/nSMase2) is a 69-74 kDa member of the neutral sphingomyelinase family of enzymes. It is a monomeric Golgi/plasma membrane enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide and phosphorylcholine. This generates second messenger components that participate in signal transduction. Human SMPD3 is a two transmembrane, 655 amino acids molecule. It contains an N-terminal luminal segment (aa 1-10), a cytoplasmic region (aa 32-64), and one catalytic domain (aa 340-646). Phosphorylation increases its MW to 78 kDa in SDS-PAGE. There is one potential isoform that possesses an Asn substitution for aa 569-587. Over aa 2-655, human SMPD3 shares 91% aa sequence identity with mouse SMPD3.
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