Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, NC‑37 human Burkitt's lymphoma B lymphoblast cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human SOCS‑6 Monoclonal Antibody (Catalog # MAB5808) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS‑6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions, using Immunoblot Buffer Group 1.
SOCS‑6 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human SOCS‑6 Monoclonal Antibody (Catalog # MAB5808) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SOCS-6 (suppressor of cytokine signaling 6; also CIS-4) is an ~60 kDa member of the SOCS family of proteins. SOCS-6 can be found in either the cytoplasm or nucleus of hematopoietic progenitors. The 535 amino acid (aa) human SOCS-6 contains one SH2 domain (aa 384‑491) and a SOCS box (aa 486‑535), which recruits E3 ubiquitin ligase complexes. It is reported to antagonize IRS-4, PI3K p85, STAT3, and other SOCS proteins, probably by promoting their degradation. SOCS-6 shares 84% and 82% aa identity with mouse and rat SOCS-6, respectively. An isoform of 418 aa diverges after aa 404.
Suppressor Of Cytokine Signaling 6
Entrez Gene IDs:
9306 (Human); 54607 (Mouse); 307200 (Rat)
CIS4CIS-4; Cytokine-inducible SH2 protein 4; HSPC060; SOCS4Cish4; SOCS6; SSI4SOCS-6; STAI4; STAT induced STAT inhibitor-4; STAT4; STATI4; Suppressor of cytokine signaling 4SOCS-4; suppressor of cytokine signaling 6
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