Human SOCS-6 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human SOCS-6 in direct ELISA and Western blot.
  • Source
    Monoclonal Mouse IgG2B Clone # 519006
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human SOCS-6
    Met1-Tyr535
    Accession # O14544
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human SOCS‑6 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, NC‑37 human Burkitt's lymphoma B lymphoblast cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human SOCS‑6 Monoclonal Antibody (Catalog # MAB5808) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS‑6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions, using Immunoblot Buffer Group 1.

Immunocytochemistry

SOCS‑6 in Jurkat Human Cell Line. SOCS‑6 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human SOCS‑6 Monoclonal Antibody (Catalog # MAB5808) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOCS-6
SOCS-6 (suppressor of cytokine signaling 6; also CIS-4) is an ~60 kDa member of the SOCS family of proteins. SOCS-6 can be found in either the cytoplasm or nucleus of hematopoietic progenitors. The 535 amino acid (aa) human SOCS-6 contains one SH2 domain (aa 384‑491) and a SOCS box (aa 486‑535), which recruits E3 ubiquitin ligase complexes. It is reported to antagonize IRS-4, PI3K p85, STAT3, and other SOCS proteins, probably by promoting their degradation. SOCS-6 shares 84% and 82% aa identity with mouse and rat SOCS-6, respectively. An isoform of 418 aa diverges after aa 404.
  • Long Name:
    Suppressor Of Cytokine Signaling 6
  • Entrez Gene IDs:
    9306 (Human); 54607 (Mouse); 307200 (Rat)
  • Alternate Names:
    CIS4CIS-4; Cytokine-inducible SH2 protein 4; HSPC060; SOCS4Cish4; SOCS6; SOCS-6; SSI4SOCS-6; STAI4; STAT induced STAT inhibitor-4; STAT4; STATI4; Suppressor of cytokine signaling 4SOCS-4; suppressor of cytokine signaling 6
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