Human SOX15 Antibody Summary
Accession # O60248
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human SOX15 by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SOX15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4070) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX15 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SOX15 in Human iPSK3 cells. SOX15 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Sheep Anti-Human SOX15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4070) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
SOX15 in A431 Human Cell Line. SOX15 was detected in immersion fixed A431 human epithelial carcinoma cell line (positive staining) and HUVEC human umbilical vein endothelial cells (negative staining) using Sheep Anti-Human SOX15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4070) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SOX15 (also known as SOX20) is a nuclear, 25 kDa transcriptional repressor that belongs to group G of the SOX gene family. Human SOX15 is 233 amino acids (aa) in length and contains a 63 aa DNA-binding HMG-box (amino acids 48‑110). Although the C-terminus (aa 126‑233) does not bind DNA, it is required for SOX15 repressive activity. It may accomplish this by interacting with beta -catenin. SOX15 is expressed in myoblasts and regulates skeletal muscle regeneration and differentiation. Human SOX15 shares 75% aa sequence identity with mouse SOX15.
Citation for Human SOX15 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/beta-catenin signaling.
Authors: Thu K, Radulovich N, Becker-Santos D, Pikor L, Pusic A, Lockwood W, Lam W, Tsao M
Sample Types: Cell Lysates
Applications: Western Blot
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