Human SPRY4 Antibody Summary
Accession # NP_112226
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human SPRY4 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human SPRY4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5070) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRY4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SPRY4 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPRY4 at approximately 45 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human SPRY4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5070) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SPRY4 (sprouty 4) is a member of the sprouty family of proteins. It blocks tyrosine kinase growth factor activation of the MAPK signaling pathway, possibly through an interaction with either Raf1 or TESK1. Human SPRY4 is 322 amino acids (aa) in length. It contains three SH3-binding domains (aa 3‑9; 25‑31; 168‑174), an NLS (aa 87‑93), a PEST sequence (aa 118‑133), and a Cys-rich/Zn++-finger domain that binds both TESK1 and Raf1 (aa 182‑298). There are two variant isoforms. One is a short form that shows an alternate start site at Met24, while a second shows a 10 aa substitution for aa 120‑129, followed by a premature truncation. Over aa 1‑177, human SPRY4 is 89% aa identical to canine SPRY4. Mouse SPRY4 is orthologous to the human short form, and shares 90% aa identity with human SPRY4.
Citation for Human SPRY4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
KSRP promotes post-transcriptional destabilization of Spry4 transcripts in non-small cell lung cancer
Authors: RK Bikkavilli, SA Zerayesus, M Van Scoyk, L Wilson, PY Wu, A Baskaran, K Tang, S Raheem, BA Samuelson, NM Reddy, SP Reddy, CD Cool, B Kosmider, S Avasarala, RA Winn
J. Biol. Chem, 2017;0(0):.
Sample Types: Cell Lysates
Applications: Western Blot
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