Human SR-AI/MSR PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2708P

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Detection of SR‑AI/MSR in THP‑1 Human Cell Line by Flow Cytometry.
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Product Details
Citations (8)
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Reviews (1)

Human SR-AI/MSR PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human SR‑AI/MSR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse SR-AI is observed.
Source
Monoclonal Mouse IgG2B Clone # 351615
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SR‑AI/MSR
Lys77-Leu451
Accession # P21757
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of SR‑AI/MSR in THP‑1 Human Cell Line by Flow Cytometry. View Larger

Detection of SR‑AI/MSR in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line activated with PMA and Ca2+ ionomycin was stained with Mouse Anti-Human SR-AI/MSR PE-conjugated Monoclonal Antibody (Catalog # FAB2708P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SR-AI/MSR

The type I class A macrophage scavenger receptor (SR-AI; also MSR-AI) is a 70-80 kDa protein that belongs to the scavenger receptor superfamily (1‑3). Receptors of this family contain characteristic extracellular domains and bind to a series of generally unrelated, but negatively-charged/polyanionic ligands (1, 3). Human SR-AI is a type II transmembrane glycoprotein that is 451 amino acids (aa) in length. It contains a 50 aa cytoplasmic tail, a 26 aa transmembrane segment and a 375 aa extracellular region (4, 5). The extracellular region contains four definitive domains, with a membrane proximal spacer of 33 aa, an alpha -helical coiled-coil domain of 163 aa, a collagen-like domain of 69 aa, and a cysteine-rich C-terminus of 110 aa (4, 6). The cysteine-rich domain (CRD) forms three intrachain disulfide bonds (7). The functional form of the molecule is a 220‑230 kDa membrane-associated trimer that, in human, apparently has two disulfide bonded chains and a third noncovalently associated subunit (8, 9). Human extracellular region is 73% and 72% aa identical to bovine and mouse SR-AI extracellular region, respectively. The human gene for SR-A gives rise to three isoforms; the I isoform of 451 aa, the II isoform of 358 aa, and the III isoform of 388 aa (4, 5, 10). All are identical through the first 344 aa which includes the cytoplasmic tail through the collagenous domain. Isoform II (SR-AII) shows a severe truncation of the CRD, but is expressed on the cell surface. Isoform III (SR-AIII) has a modest truncation of the CRD, and cannot be expressed on the cell surface. However, relative to SR-AI, SR-AII is known to show differential sensitivity to LPS and receptor binding to gram‑negative bacteria (9, 11), while SR-AIII is known to be a dominant-negative isoform (10). SR-AIII may achieve this by either heterotrimerizing with SR-AI, or simply eliminating the production of SR-AI mRNA.

References
  1. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  2. Linton, M.F. and S. Fazio (2001) Curr. Opin. Lipidol. 12:489.
  3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  4. Matsumoto, A. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9133.
  5. Emi, M. et al. (1993) J. Biol. Chem. 268:2120.
  6. Naito, M. et al. (1992) Am. J. Pathol. 141:591.
  7. Resnick, D. et al. (1996) J. Biol. Chem. 271:26924.
  8. Ashkenas, J. et al. (1993) J. Lipid Res. 34:983.
  9. Penman, M. et al. (1991) J. Biol. Chem. 266:23985.
  10. Gough, P.J. et al. (1998) J. Lipid Res. 39:531.
  11. Peiser, L. et al. (2000) Inf. Immun. 68:1953.
Long Name
Macrophage Scavenger Receptor Types I and II
Entrez Gene IDs
4481 (Human); 20288 (Mouse); 25073 (Rat)
Alternate Names
CD204 antigen; CD204; Macrophage acetylated LDL receptor I and II; macrophage scavenger receptor 1; macrophage scavenger receptor type III; MSR1; phSR1; phSR2; SCARA1; SCARA1macrophage scavenger receptor types I and II; Scavenger receptor class A member 1; scavenger receptor class A, member 1; SR-A; SRAI; SR-AI

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Citations for Human SR-AI/MSR PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Gastric cancer-derived mesenchymal stromal cells trigger M2 macrophage polarization that promotes metastasis and EMT in gastric cancer
    Authors: W Li, X Zhang, F Wu, Y Zhou, Z Bao, H Li, P Zheng, S Zhao
    Cell Death Dis, 2019;10(12):918.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Oxidized LDL triggers changes in oxidative stress and inflammatory biomarkers in human macrophages
    Authors: OJ Lara-Guzmá, Á Gil-Izquie, S Medina, E Osorio, R Álvarez-Qu, N Zuluaga, C Oger, JM Galano, T Durand, K Muñoz-Dura
    Redox Biol, 2017;15(0):1-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The Impact of Protein Corona Formation on the Macrophage Cellular Uptake and Biodistribution of Spherical Nucleic Acids
    Authors: AB Chinen, CM Guan, CH Ko, CA Mirkin
    Small, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer
    Authors: B Zhang, M Cao, Y He, Y Liu, G Zhang, C Yang, Y Du, J Xu, J Hu, F Gao
    J Cancer, 2017;8(17):3522-3530.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Increased circulating M2-like monocytes in patients with breast cancer
    Authors: B Zhang, M Cao, Y He, Y Liu, G Zhang, C Yang, Y Du, J Xu, J Hu, F Gao
    Tumour Biol., 2017;39(6):1010428317711.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages
    Authors: F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Oxidative stress decreases functional airway mannose binding lectin in COPD.
    Authors: Tran, Hai B, Ahern, Jessica, Hodge, Greg, Holt, Phillip, Dean, Melinda, Reynolds, Paul N, Hodge, Sandra
    PLoS ONE, 2014;9(6):e98571.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Effect of apoptotic cell recognition on macrophage polarization and mycobacterial persistence.
    Authors: de Oliveira Fulco T, Andrade P, de Mattos Barbosa M, Pinto T, Ferreira P, Ferreira H, da Costa Nery J, Real S, Borges V, Moraes M, Sarno E, Sampaio E, Pinheiro R
    Infect Immun, 2014;82(9):3968-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human SR-AI/MSR PE-conjugated Antibody
By Anonymous on 11/17/2021
Application: Flow Sample Tested: Macrophages,Peritoneal macrophages Species: Human