Human SR-AI/MSR PE-conjugated Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human SR‑AI/MSR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse SR-AI is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 351615
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human SR‑AI/MSR
    Lys77-Leu451
    Accession # P21757
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SR‑AI/MSR in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line activated with PMA and Ca2+ ionomycin was stained with Mouse Anti-Human SR‑AI/MSR PE-conjugated Monoclonal Antibody (Catalog # FAB2708P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SR-AI/MSR

The type I class A macrophage scavenger receptor (SR-AI; also MSR-AI) is a 70-80 kDa protein that belongs to the scavenger receptor superfamily (1‑3). Receptors of this family contain characteristic extracellular domains and bind to a series of generally unrelated, but negatively-charged/polyanionic ligands (1, 3). Human SR-AI is a type II transmembrane glycoprotein that is 451 amino acids (aa) in length. It contains a 50 aa cytoplasmic tail, a 26 aa transmembrane segment and a 375 aa extracellular region (4, 5). The extracellular region contains four definitive domains, with a membrane proximal spacer of 33 aa, an alpha -helical coiled-coil domain of 163 aa, a collagen-like domain of 69 aa, and a cysteine-rich C-terminus of 110 aa (4, 6). The cysteine-rich domain (CRD) forms three intrachain disulfide bonds (7). The functional form of the molecule is a 220‑230 kDa membrane-associated trimer that, in human, apparently has two disulfide bonded chains and a third noncovalently associated subunit (8, 9). Human extracellular region is 73% and 72% aa identical to bovine and mouse SR-AI extracellular region, respectively. The human gene for SR-A gives rise to three isoforms; the I isoform of 451 aa, the II isoform of 358 aa, and the III isoform of 388 aa (4, 5, 10). All are identical through the first 344 aa which includes the cytoplasmic tail through the collagenous domain. Isoform II (SR-AII) shows a severe truncation of the CRD, but is expressed on the cell surface. Isoform III (SR-AIII) has a modest truncation of the CRD, and cannot be expressed on the cell surface. However, relative to SR-AI, SR-AII is known to show differential sensitivity to LPS and receptor binding to gram‑negative bacteria (9, 11), while SR-AIII is known to be a dominant-negative isoform (10). SR-AIII may achieve this by either heterotrimerizing with SR-AI, or simply eliminating the production of SR-AI mRNA.

  • References:
    1. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
    2. Linton, M.F. and S. Fazio (2001) Curr. Opin. Lipidol. 12:489.
    3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
    4. Matsumoto, A. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9133.
    5. Emi, M. et al. (1993) J. Biol. Chem. 268:2120.
    6. Naito, M. et al. (1992) Am. J. Pathol. 141:591.
    7. Resnick, D. et al. (1996) J. Biol. Chem. 271:26924.
    8. Ashkenas, J. et al. (1993) J. Lipid Res. 34:983.
    9. Penman, M. et al. (1991) J. Biol. Chem. 266:23985.
    10. Gough, P.J. et al. (1998) J. Lipid Res. 39:531.
    11. Peiser, L. et al. (2000) Inf. Immun. 68:1953.
  • Long Name:
    Macrophage Scavenger Receptor Types I and II
  • Entrez Gene IDs:
    4481 (Human); 20288 (Mouse); 25073 (Rat)
  • Alternate Names:
    CD204 antigen; CD204; Macrophage acetylated LDL receptor I and II; macrophage scavenger receptor 1; macrophage scavenger receptor type III; MSR1; phSR1; phSR2; SCARA1; SCARA1macrophage scavenger receptor types I and II; Scavenger receptor class A member 1; scavenger receptor class A, member 1; SR-A; SRAI; SR-AI
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages
    Authors: F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Oxidative stress decreases functional airway mannose binding lectin in COPD.
    Authors: Tran, Hai B, Ahern, Jessica, Hodge, Greg, Holt, Phillip, Dean, Melinda, Reynolds, Paul N, Hodge, Sandra
    PLoS ONE, 2014;9(6):e98571.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Effect of apoptotic cell recognition on macrophage polarization and mycobacterial persistence.
    Authors: de Oliveira Fulco T, Andrade P, de Mattos Barbosa M, Pinto T, Ferreira P, Ferreira H, da Costa Nery J, Real S, Borges V, Moraes M, Sarno E, Sampaio E, Pinheiro R
    Infect Immun, 2014;82(9):3968-78.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 20  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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