Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-reported

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 351615
View all SR-AI/MSR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SR‑AI/MSR
Lys77-Leu451
Accession # P21757

Specificity

Detects human SR‑AI/MSR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse SR-AI is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human SR‑AI/MSR Antibody

Detection of Human SR-AI/MSR antibody by Western Blot.

Detection of Human SR‑AI/MSR by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 72 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SR-AI/MSR Monoclonal Antibody (Catalog # MAB2708) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SR-AI/MSR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of SR‑AI/MSR in M2 macrophages cells by Flow Cytometry.

M2 macrophages cells treated with 50 ng/mL Recombinant Human M-CSF Protein (216-MC) for 6 days followed by an overnight polarization with 20 ng/mL Recombinant Human IL-4 Protein (204-IL) and 20 ng/mL Recombinant Human IL-13 Protein (213-ILB) were stained with Mouse Anti-Human SR‑AI/MSR Monoclonal Antibody (Catalog # MAB2708, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human SR‑AI/MSR Antibody

Application
Recommended Usage

CyTOF-reported

This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: M2 macrophages cells treated with 50 ng/mL Recombinant Human M-CSF Protein (Catalog # 216-MC) for 6 days followed by an overnight polarization with 20 ng/mL Recombinant Human IL-4 Protein (Catalog # 204-IL) and 20 ng/mL Recombinant Human IL-13 Protein (Catalog # 213-ILB) 

Western Blot

1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line treated with PMA

Reviewed Applications

Read 1 review rated 5 using MAB2708 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SR-AI/MSR

The type I class A macrophage scavenger receptor (SR-AI; also MSR-AI) is a 70-80 kDa protein that belongs to the scavenger receptor superfamily (1‑3). Receptors of this family contain characteristic extracellular domains and bind to a series of generally unrelated, but negatively-charged/polyanionic ligands (1, 3). Human SR-AI is a type II transmembrane glycoprotein that is 451 amino acids (aa) in length. It contains a 50 aa cytoplasmic tail, a 26 aa transmembrane segment and a 375 aa extracellular region (4, 5). The extracellular region contains four definitive domains, with a membrane proximal spacer of 33 aa, an alpha -helical coiled-coil domain of 163 aa, a collagen-like domain of 69 aa, and a cysteine-rich C-terminus of 110 aa (4, 6). The cysteine-rich domain (CRD) forms three intrachain disulfide bonds (7). The functional form of the molecule is a 220‑230 kDa membrane-associated trimer that, in human, apparently has two disulfide bonded chains and a third noncovalently associated subunit (8, 9). Human extracellular region is 73% and 72% aa identical to bovine and mouse SR-AI extracellular region, respectively. The human gene for SR-A gives rise to three isoforms; the I isoform of 451 aa, the II isoform of 358 aa, and the III isoform of 388 aa (4, 5, 10). All are identical through the first 344 aa which includes the cytoplasmic tail through the collagenous domain. Isoform II (SR-AII) shows a severe truncation of the CRD, but is expressed on the cell surface. Isoform III (SR-AIII) has a modest truncation of the CRD, and cannot be expressed on the cell surface. However, relative to SR-AI, SR-AII is known to show differential sensitivity to LPS and receptor binding to gram‑negative bacteria (9, 11), while SR-AIII is known to be a dominant-negative isoform (10). SR-AIII may achieve this by either heterotrimerizing with SR-AI, or simply eliminating the production of SR-AI mRNA.

References

  1. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  2. Linton, M.F. and S. Fazio (2001) Curr. Opin. Lipidol. 12:489.
  3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  4. Matsumoto, A. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9133.
  5. Emi, M. et al. (1993) J. Biol. Chem. 268:2120.
  6. Naito, M. et al. (1992) Am. J. Pathol. 141:591.
  7. Resnick, D. et al. (1996) J. Biol. Chem. 271:26924.
  8. Ashkenas, J. et al. (1993) J. Lipid Res. 34:983.
  9. Penman, M. et al. (1991) J. Biol. Chem. 266:23985.
  10. Gough, P.J. et al. (1998) J. Lipid Res. 39:531.
  11. Peiser, L. et al. (2000) Inf. Immun. 68:1953.

Long Name

Macrophage Scavenger Receptor Types I and II

Alternate Names

CD204, MSR1, SCARA1, SRAI

Entrez Gene IDs

4481 (Human); 20288 (Mouse); 25073 (Rat)

Gene Symbol

MSR1

UniProt

P21757

Additional SR-AI/MSR Products

Product Documents for Human SR‑AI/MSR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SR‑AI/MSR Antibody

For research use only

Citations for Human SR‑AI/MSR Antibody

Customer Reviews for Human SR‑AI/MSR Antibody (1)

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  • Human SR-AI/MSR Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 03/26/2022
    Lysate of THP-1 human acute monocytic leukemia cell line treated with 80nM PMA for 72 hours.
    Human SR‑AI/MSR Antibody MAB2708

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Associated Pathways

A-beta Pathways: Uptake & Degradation A-beta Pathways: Uptake & Degradation Thumbnail