Human SR-AI/MSR Antibody

Catalog # Availability Size / Price Qty
MAB2708
MAB2708-SP
Human SR-AI/MSR Antibody in Western Blot
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Product Details
Citations (1)
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Human SR-AI/MSR Antibody Summary

Species Reactivity
Human
Specificity
Detects human SR‑AI/MSR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse SR-AI is observed.
Source
Monoclonal Mouse IgG2B Clone # 351615
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SR‑AI/MSR
Lys77-Leu451
Accession # P21757
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
THP‑1 human acute monocytic leukemia cell line treated with PMA and Ca2+ ionomycin
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human SR‑AI/MSR by Western Blot. View Larger

Detection of Human SR‑AI/MSR by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 72 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SR-AI/MSR Monoclonal Antibody (Catalog # MAB2708) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SR-AI/MSR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SR-AI/MSR

The type I class A macrophage scavenger receptor (SR-AI; also MSR-AI) is a 70-80 kDa protein that belongs to the scavenger receptor superfamily (1‑3). Receptors of this family contain characteristic extracellular domains and bind to a series of generally unrelated, but negatively-charged/polyanionic ligands (1, 3). Human SR-AI is a type II transmembrane glycoprotein that is 451 amino acids (aa) in length. It contains a 50 aa cytoplasmic tail, a 26 aa transmembrane segment and a 375 aa extracellular region (4, 5). The extracellular region contains four definitive domains, with a membrane proximal spacer of 33 aa, an alpha -helical coiled-coil domain of 163 aa, a collagen-like domain of 69 aa, and a cysteine-rich C-terminus of 110 aa (4, 6). The cysteine-rich domain (CRD) forms three intrachain disulfide bonds (7). The functional form of the molecule is a 220‑230 kDa membrane-associated trimer that, in human, apparently has two disulfide bonded chains and a third noncovalently associated subunit (8, 9). Human extracellular region is 73% and 72% aa identical to bovine and mouse SR-AI extracellular region, respectively. The human gene for SR-A gives rise to three isoforms; the I isoform of 451 aa, the II isoform of 358 aa, and the III isoform of 388 aa (4, 5, 10). All are identical through the first 344 aa which includes the cytoplasmic tail through the collagenous domain. Isoform II (SR-AII) shows a severe truncation of the CRD, but is expressed on the cell surface. Isoform III (SR-AIII) has a modest truncation of the CRD, and cannot be expressed on the cell surface. However, relative to SR-AI, SR-AII is known to show differential sensitivity to LPS and receptor binding to gram‑negative bacteria (9, 11), while SR-AIII is known to be a dominant-negative isoform (10). SR-AIII may achieve this by either heterotrimerizing with SR-AI, or simply eliminating the production of SR-AI mRNA.

References
  1. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  2. Linton, M.F. and S. Fazio (2001) Curr. Opin. Lipidol. 12:489.
  3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  4. Matsumoto, A. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9133.
  5. Emi, M. et al. (1993) J. Biol. Chem. 268:2120.
  6. Naito, M. et al. (1992) Am. J. Pathol. 141:591.
  7. Resnick, D. et al. (1996) J. Biol. Chem. 271:26924.
  8. Ashkenas, J. et al. (1993) J. Lipid Res. 34:983.
  9. Penman, M. et al. (1991) J. Biol. Chem. 266:23985.
  10. Gough, P.J. et al. (1998) J. Lipid Res. 39:531.
  11. Peiser, L. et al. (2000) Inf. Immun. 68:1953.
Long Name
Macrophage Scavenger Receptor Types I and II
Entrez Gene IDs
4481 (Human); 20288 (Mouse); 25073 (Rat)
Alternate Names
CD204 antigen; CD204; Macrophage acetylated LDL receptor I and II; macrophage scavenger receptor 1; macrophage scavenger receptor type III; MSR1; phSR1; phSR2; SCARA1; SCARA1macrophage scavenger receptor types I and II; Scavenger receptor class A member 1; scavenger receptor class A, member 1; SR-A; SRAI; SR-AI

Product Datasheets

Citation for Human SR-AI/MSR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages.
    Authors: Hongo S, Watanabe T, Arita S
    Am. J. Physiol. Endocrinol. Metab., 2009;297(2):E474-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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