Detection of STYK1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human STYK1 PE‑conjugated Monoclonal Antibody (Catalog # IC6668P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
STYK1 (Ser/Thr/Tyr kinase 1) also known as Novel Oncogene with Kinase-domain (NOK), is a 46-50 kDa, member of the FGFR/PDGFR (or RPTK-like) family of tyrosine kinases. It appears to be a transmembrane (TM) component of early endosomes, and likely participates in the trafficking of cell membrane receptors such as the EGFR. STYK1 appears to form multimers, a process regulated by its TM domain. Cells recognized to express STYK1 are principally tumor cellls, as normal cell expression seems to be restricted, and shows little correlation between mice and human. Both MAPK and PI3K pathways are activated by STYK1 activity, and STYK1 is associated with oncogenesis. Human STYK1 is 422 amino acids (aa) in length. It contains 25 aa N-terminus, a 21 aa putative transmembrane segment, and a 396 aa C-terminus that contains a Tyr-kinase domain (aa 118-372). Over aa 72-320, human STYK1 is 77% and 83% identical to mouse and canine STYK1, respectively.
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