Human STYK1 PE-conjugated Antibody

IC6668P has been discontinued. View all STYK1 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human STYK1 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 484713
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human STYK1
    Asp72-Val320
    Accession # Q6J9G0
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of STYK1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human STYK1 PE‑conjugated Monoclonal Antibody (Catalog # IC6668P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: STYK1
STYK1 (Ser/Thr/Tyr kinase 1) also known as Novel Oncogene with Kinase-domain (NOK), is a 46-50 kDa, member of the FGFR/PDGFR  (or RPTK-like) family of tyrosine kinases.  It appears to be a transmembrane (TM) component of early endosomes, and likely participates in the trafficking of cell membrane receptors such as the EGFR. STYK1 appears to form multimers, a process regulated by its TM domain. Cells recognized to express STYK1 are principally tumor cellls, as normal cell expression seems to be restricted, and shows little correlation between mice and human. Both MAPK and PI3K pathways are activated by STYK1 activity, and STYK1 is associated with oncogenesis.  Human STYK1 is 422 amino acids (aa) in length.  It contains 25 aa N-terminus, a 21 aa putative transmembrane segment, and a 396 aa C-terminus that contains a Tyr-kinase domain (aa 118-372).  Over aa 72-320, human STYK1 is 77% and 83% identical to mouse and canine STYK1, respectively.  
  • Long Name:
    Serine/Threonine/Tyrosine Kinase 1
  • Entrez Gene IDs:
    55359 (Human); 243659 (Mouse); 500340 (Rat)
  • Alternate Names:
    DKFZp761P1010; EC 2.7.10.2; NOK; NOKProtein PK-unique; serine/threonine/tyrosine kinase 1Novel oncogene with kinase domain; STYK1; SuRTK106; tyrosine-protein kinase STYK1
Related Research Areas

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