Human SUMO1-Specific Peptidase 1/
SENP1 Antibody

Catalog # Availability Size / Price Qty
AF6587
AF6587-SP
Human SUMO1-Specific Peptidase 1/SENP1 Antibody in Data
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Human SUMO1-Specific Peptidase 1/
SENP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SUMO1-Specific Peptidase 1/SENP1 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SUMO1-Specific Peptidase 1/SENP1
Glu419-Leu644
Accession # Q9P0U3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry SUMO-Specific Peptidase 1/SENP1 antibody in T47D Human Cell Line by Immunocytochemistry (ICC). View Larger

SUMO‑Specific Peptidase 1/SENP1 in T47D Human Cell Line. SUMO-Specific Peptidase 1/SENP1 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human SUMO-Specific Peptidase 1/SENP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6587) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry SUMO1-Specific Peptidase 1/SENP1 antibody in Human Testis by Immunohistochemistry (IHC-P). View Larger

SUMO1-Specific Peptidase 1/SENP1 in Human Testis. SUMO1-Specific Peptidase 1/SENP1 was detected in immersion fixed paraffin-embedded sections of human testis using Sheep Anti-Human SUMO1-Specific Peptidase 1/SENP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6587) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to late spermatids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SENP1

SENP1 (Sentrin/SUMO-specific protease) is a member of the SENP family of proteases. Sentrin/SUMO-specific proteases (SENPs) are a group of cysteine-type peptidases that catalyze two essential functions in the SUMO pathways: processing of full-length SUMOs to their mature forms and deconjugation of SUMOs from SUMOlyated proteins. The seven mammalian SENPs share a conserved C-terminal catalytic domain while the N-terminal domains have no significant similarity. Human SENP-1 has broad specificity for the three mammalian SUMOs. It is found in the cytoplasm and nucleus depending on cell type, and is expressed in testis, thymus, pancreas, spleen, liver, ovary and small intestine. It is thought that localization of SENP-1 is vital for the regulation for SUMOylation status of target proteins. The small ubiquitin-like modifier (SUMO) is a member of ubiquitin-like protein family. SUMO modification of the target proteins is a reversible process that regulates many cellular processes including transcription regulation, nuclear localization, centromere segregation and signal transduction. The recombinant human SENP-1 (immunogen) includes the catalytic domain, which has been shown to be sufficient for SENP-1 activity and substrate specificity.

Long Name
SUMO-Specific Peptidase 1
Entrez Gene IDs
29843 (Human); 223870 (Mouse); 300193 (Rat)
Alternate Names
EC 3.4.22; EC 3.4.22.-; SENP1; Sentrin/SUMO-specific protease SENP1; sentrin-specific protease 1; SUMO1/sentrin specific peptidase 1; SUMO1/sentrin specific protease 1; SuPr-2

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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SENP1 Antibody

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