Human Syndecan-2/CD362 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB2965R-100
MAB2965R-SP
MAB2965R-500
MAB2965R-01M
Detection of  Syndecan-2/CD362 in Human PBMCs by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Syndecan-2/CD362 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Syndecan-2/CD362 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Syndecan-1, recombinant human (rh) Syndecan-3, or rhSyndecan-4 is observed.
Source
Recombinant Monoclonal Rat IgG2B Clone # 305515R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Syndecan-2/CD362
Glu19-Gly144
Accession # AAH49836
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of  Syndecan-2/CD362 in Human PBMCs by Flow Cytometry. View Larger

Detection of  Syndecan-2/CD362 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Rat Anti-Human Syndecan-2/CD362 Monoclonal Antibody (Catalog # MAB2965R) or (B) Rat IgG2B Isotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0102B). 

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-2/CD362

Syndecan-2, previously known as fibroglycan or heparan sulfate proteoglycan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan-2 is most similar to Syndecan-4 (1‑3). Human Syndecan-2 is synthesized as a 201 amino acid (aa) core protein with an 18 aa signal sequence, a 126 aa extracellular domain (ECD), a 25 aa transmembrane region and a 32 aa cytoplasmic tail (4). The human ECD of Syndecan-2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 73%, 87%, 78% and 63% aa identity with the ECD of mouse, rat, bovine, canine and chicken Syndecan-2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 22 kDa core protein. Non-covalent homodimerization of Syndecan-2 is dependent on the transmembrane domain (5). Syndecan-2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is upregulated in several cancer cell lines (6). After induction in macrophages by inflammatory mediators, Syndecan-2 selectively binds FGFbasic, VEGF and EGF (7). Syndecan-2 expressed on human primary osteoblasts binds GM-CSF and may function as a co‑receptor (8). Activated endothelial cell Syndecan-2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient (9). Typically, cytokine, chemokine and extracellular matrix protein binding occurs through interaction with HS side chains, but the Syndecan-2 extracellular domain can bind TGF-beta directly via protein-protein interaction (10).

References
  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
  3. Essner, J. J. et al. (2006) Int. J. Biochem. Cell Biol. 38:152.
  4. Marynen, P. et al. (1989) J. Biol. Chem. 264:7017.
  5. Choi, S. et al. (2005) J. Biol. Chem. 280:42573.
  6. Park, H. et al. (2002) J. Biol. Chem. 277:29730.
  7. Clasper, S. et al. (1999) J. Biol. Chem. 274:24113.
  8. Modrowski, D. et al. (2000) J. Biol. Chem. 275:9178.
  9. Halden, Y. et al. (2004) Biochem. J. 377:533.
  10. Chen, L. et al. (2004) J. Biol. Chem. 279:15715.
Entrez Gene IDs
6383 (Human); 15529 (Mouse)
Alternate Names
CD362 antigen; CD362; Fibroglycan; heparan sulfate proteoglycan 1, cell surface-associated; Heparan sulfate proteoglycan core protein; HSPG; HSPG1; HSPG1syndecan proteoglycan 2; SDC2; SYND2; SYND2cell surface-associated heparan sulfate proteoglycan 1; syndecan 2; Syndecan2; Syndecan-2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Syndecan-2/CD362 Antibody

There are currently no reviews for this product. Be the first to review Human Syndecan-2/CD362 Antibody and earn rewards!

Have you used Human Syndecan-2/CD362 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review