Human TBK1 Antibody

Catalog # Availability Size / Price Qty
AF9934-100
AF9934-SP
TBK1 in  Human Prostate Cancer Tissue.
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Human TBK1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TBK-1 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TBK-1
Met1-Gly121
Accession # Q9UHD2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry TBK1 in  Human Prostate Cancer Tissue. View Larger

TBK1 in Human Prostate Cancer Tissue. TBK1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human TBK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9934) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBK1

TANK binding kinase-1 (TBK1), encoded by the TBK1 gene, is a multimeric Serine/threonine-protein kinase that mediates a crosstalk between the innate immune response and autophagy. TBK1 was first identified as a TANK-interacting protein controlling NF-kappa B-mediated responses. Mutations in TBK1 have been found in conditions like amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), normal tension glaucoma (NTG) and childhood herpes simplex encephalitis (HSE). Gain-of-function of TBK1 are associated with NTG, whereas loss-of-function mutations result in ALS/FTD or in HSE.

Long Name
TANK Binding Kinase 1
Entrez Gene IDs
29110 (Human); 56480 (Mouse); 299827 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; FLJ11330; FTDALS4; NAK serine/threonine-protein kinase TBK 1; NAK; NAKserine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; NFKB-Activating Kinase; T2K; TANK-binding kinase 1NF-kB-activating kinase; TBK 1; TBK1

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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